Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves

MSystems
Samat AmatEdouard Timsit

Abstract

Six Lactobacillus strains originating from the nasopharyngeal microbiota of cattle were previously characterized in vitro and identified as candidate bacterial therapeutics (BTs) for mitigating the bovine respiratory pathogen Mannheimia haemolytica In the present study, these BT strains were evaluated for their potential to (i) reduce nasal colonization by M. haemolytica, (ii) modulate the nasal microbiota, and (iii) stimulate an immune response in calves experimentally challenged with M. haemolytica. Twenty-four Holstein bull calves (1 to 3 weeks old) received either an intranasal BT cocktail containing 6 Lactobacillus strains (3 × 109 CFU per strain; BT + Mh group) 24 h prior to intranasal M. haemolytica challenge (3 × 108 CFU) or no BTs prior to challenge (Mh, control group). Nasal swab, blood, and transtracheal aspiration samples were collected over the course of 16 days after BT inoculation. Counts of M. haemolytica were determined by culturing, and the nasal and tracheal microbiotas were evaluated using 16S rRNA gene sequencing. Serum cytokines (interleukin-6 [IL-6], IL-8, and IL-10) were quantified by enzyme-linked immunosorbent assay (ELISA). Administration of BT reduced nasal colonization by M. haemolytica (P = 0.02), ...Continue Reading

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Citations

Mar 9, 2021·Animal Health Research Reviews·Trevor W AlexanderSamat Amat
Sep 11, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·María Hortencia MirandaMaría Elena Fátima Nader-Macías

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Datasets Mentioned

BETA
PRJNA589656

Methods Mentioned

BETA
PCR
electrophoresis
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

R
SAS
DESeq2
PERMANOVA
R packages vegan

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