Intranasal immunization with C5a peptidase prevents nasopharyngeal colonization of mice by the group A Streptococcus.

Infection and Immunity
Y JiP Patrick Cleary

Abstract

Early inflammatory events are initiated by phased production of C5a and interleukin-8 in tissue. Most serotypes of group A streptococci express a surface-bound peptidase (SCPA) which specifically cleaves mouse and human C5a chemotaxins. This study investigates the impact of SCPA on colonization of the nasopharyngeal mucosa of mice and evaluates its potential to induce protective immunity. Two strains, serotypes M6 and M49, which contain insertion and deletion mutations in the SCPA gene (scpA) and represent the two major subdivisions of group A streptococci, were characterized and compared in a mouse intranasal infection model. In this model, SCPA mutants were more rapidly cleared from the nasopharynges of inoculated mice compared with wild-type strains. A 2,908-bp fragment of scpA49 gene, obtained by PCR, was ligated to the expression vector pGEX-4T-1 and expressed in Escherichia coli. The affinity-purified deltaSCPA49 protein proved to be highly immunogenic in mice and rabbits. Although the purified deltaSCPA49 immunogen lacked enzymatic activity, it induced high titers of rabbit antibodies which were able to neutralize peptidase activity associated with M1, M6, M12, and M49 streptococci in vitro. This result confirmed that an...Continue Reading

References

Oct 1, 1992·Infection and Immunity·P P ClearyP Ferrieri
Dec 1, 1992·Infection and Immunity·P P ClearyJ Handley
Feb 1, 1992·The Journal of Investigative Dermatology·S A BoothR D Nelson
Jan 1, 1991·The Journal of Infectious Diseases·S P O'ConnorP P Cleary
Jul 1, 1989·Clinical Microbiology Reviews·V A Fischetti
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D E WexlerP P Cleary
Jun 1, 1981·Infection and Immunity·C G GemmellP G Quie
Jan 1, 1995·Infection and Immunity·A PodbielskiJ Weber-Heynemann
May 1, 1993·Molecular Microbiology·S K HollingsheadD E Bessen
Jun 1, 1993·Infection and Immunity·S K HollingsheadS M Michalek
Apr 1, 1996·The Journal of Infectious Diseases·D E BessenS K Hollingshead
Jul 1, 1996·Infection and Immunity·I ChmouryguinaP P Cleary

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Citations

Jun 12, 1998·Pediatric Annals·J B Dale
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hae-Sun ParkP Patrick Cleary
Sep 7, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ronit Cohen-Poradosu, Dennis L Kasper
May 31, 2008·Current Infectious Disease Reports·Karen L Kotloff
Mar 1, 2008·Proteomics. Clinical Applications·Jason N ColeMark J Walker
Feb 17, 2017·Journal of the Pediatric Infectious Diseases Society·Nicholas D HysmithJames B Dale
Jan 18, 2005·Clinical Microbiology Reviews·Gunnar LindahlThomas Areschoug
Jun 10, 2009·Infection and Immunity·Ute Gleich-TheurerBarbara Spellerberg
Apr 4, 2014·Clinical Microbiology Reviews·Mark J WalkerVictor Nizet
Feb 24, 2012·Cell and Tissue Research·Joanna Koziel, Jan Potempa
Jan 15, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Misty L KuhnGuido Grandi
Jun 22, 1999·Immunologic Research·E R Brandt, M F Good
Sep 16, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alan L BisnoUNKNOWN National Institute of Allergy and Infectious Diseases
Apr 18, 2000·Infection and Immunity·D A ScaramuzzinoD E Bessen
Oct 13, 2006·Current Opinion in Infectious Diseases·R F BreimanP M McInnes
Feb 26, 2004·Indian Journal of Pediatrics·Anita Shet, Edward Kaplan
Jun 3, 2009·Expert Review of Vaccines·Melina M GeorgousakisKadaba S Sriprakash
May 21, 2011·Clinical Reviews in Allergy & Immunology·Christopher Chang
Oct 3, 2009·Current Opinion in Infectious Diseases·Andrew C SteerJonathan R Carapetis
May 20, 2011·The Journal of Infectious Diseases·Ya-Shu HuangZehava Eichenbaum
Apr 4, 2002·Molecular Microbiology·Magnus Rasmussen, Lars Björck
Oct 26, 2002·Journal of Bacteriology·Irina V KorolevaAlexander N Suvorov
Mar 27, 2002·Journal of Bacteriology·Timothy C Barnett, June R Scott
Aug 31, 2006·The Pediatric Infectious Disease Journal·Thomas B MartinsHarry R Hill
Jun 26, 2012·Current Infectious Disease Reports·Manisha PandeyMichael F Good
Jun 24, 2015·Journal of Immunology Research·Shu Ki TsoiAndrew C Steer
Jul 19, 2013·Human Vaccines & Immunotherapeutics·Michael F GoodManisha Pandey

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