Intranasal immunization with live attenuated influenza vaccine plus chitosan as an adjuvant protects mice against homologous and heterologous virus challenge

Archives of Virology
Xueliang WangZe Chen

Abstract

Our previous studies have proven the adjuvanticity of chitosan in mice when administered with inactivated and subunit influenza vaccine. In this study, we investigated the adjuvant effect of chitosan on the immunogenicity and protective efficacy of a live attenuated influenza vaccine. Mice were inoculated intranasally with live attenuated influenza vaccine plus chitosan and then challenged with a high, lethal dose of homologous or heterologous virus. Antibody responses, secretion of IFN-γ by spleen cells, body weight loss, survival rates, and residual lung virus titers were tested. The results demonstrated that live attenuated influenza vaccine with chitosan adjuvant not only protected mice completely against challenge with the homologous virus but also provided good cross-protection against a heterologous virus. In addition, chitosan as adjuvant could significantly increase the levels of antigen-specific antibodies and the population of IFN-γ-secreting T cells. These results reveal the potential of chitosan as a candidate adjuvant for use in a live attenuated influenza vaccine.

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Citations

Dec 18, 2013·AAPS PharmSciTech·Chompoonuch SawaengsakNuttanan Sinchaipanid
Jun 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·José V Pérez-GirónCésar Muñoz-Fontela
Dec 3, 2016·Avian Diseases·Arithat LimsatanunSomsak Pakpinyo
Oct 18, 2015·Applied Microbiology and Biotechnology·Xueliang WangHualiang Wang
May 1, 2021·International Journal of Biological Macromolecules·Mehrnoush SafarzadehMostafa Haji Molla Hoseini

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