Intranasal vaccination of rabbits with Pasteurella multocida A:3 outer membranes that express iron-regulated proteins

American Journal of Veterinary Research
Anthony W ConferG L Meredith

Abstract

To determine efficacy of intranasal vaccination of rabbits with Pasteurella multocida A:3 outer membrane proteins (OMP) expressing iron-regulated OMP (IROMP) in conferring protection against experimental challenge exposure. 52 male New Zealand White rabbits. Rabbits were vaccinated intranasally on days 0, 7, and 14; some vaccines included cholera toxin (CT) as an adjuvant. Concentrations of intranasal IgA and serum IgG antibodies against P multocida OMP were determined. In experiment A, rabbits were vaccinated with either phospate-buffered saline solution (PBSS), PBSS-CT, OMP-CT, or IROMP-CT, challenge-exposed intranasally on day 16, and euthanatized and necropsied on day 28. Rabbits were also vaccinated with OMP or IROMP without CT and were not challenge-exposed. In experiment B, rabbits were vaccinated with PBSS, PBSS-CT, IROMP, or IROMP-CT. On day 17, rabbits were challenge-exposed intranasally. Nasal bacteria and antibodies were determined on day 24. In experiment A, OMP-CT vaccination stimulated mucosal and systemic antibody responses to the bacterium and enhanced resistance against challenge exposure. Intranasal bacterial counts were not significantly reduced. Vaccination with IROMP-CT stimulated mucosal and systemic anti...Continue Reading

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Citations

Jul 3, 2007·The Annals of Occupational Hygiene·Shannon H Gaffney, Dennis J Paustenbach
Nov 19, 2003·American Journal of Veterinary Research·Misty C JordanRonald J Martens
Dec 4, 2013·Vaccine·Tarek A AhmadLaila H El-Sayed
Jul 5, 2013·Clinical Microbiology Reviews·Brenda A Wilson, Mengfei Ho
Jan 15, 2015·TheScientificWorldJournal·A PrasannavadhanaVijendra Pal Singh
Aug 10, 2017·Molecular Medicine Reports·Huihui DuZeyang Zhou

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