Abstract
Helper-dependent adenoviral (HDAd) vectors were developed primarily for genetic disease therapy by deleting all coding regions for attenuating the host cellular immune response to adenovirus (Ad) and long-lasting gene expression. Recently Harui et al. reported that HDAd vaccine could stimulate superior transgene-specific cytotoxic T lymphocyte (CTL) and antibody responses via the intraperitoneal route, compared to first-generation adenoviral (FGAd) vaccine. This prompted us to explore the potential of HDAd as a vaccine vector administrated intranasally. In this study, we prepared HDAd and FGAd vectors expressing enhanced green fluorescent protein (EGFP), respectively, and compared their efficacy in mice. Mice were immunized intranasally with 5x10(9) vp HDAd or FGAd vector particles. Despite stimulating similar anti-Ad antibody responses with FGAd vaccine in the prime/boost strategy, HDAd vector expressing EGFP displayed superior transgene-specific serum IgG, mucosal IgA and cellular immune response, with the characterization of balanced or mixed Th1/Th2 CD4+ T-cell responses. Meanwhile, a single dose of intranasal (i.n.) vaccine of HDAd-EGFP induced a serum IgG response with more efficacy than FGAd-EGFP. In addition, i.n. boost...Continue Reading
References
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Sep 1, 1993·The Journal of Infectious Diseases·W S GallichanK L Rosenthal
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·R J ParksF L Graham
Apr 16, 1998·Gene Therapy·M A MorsyC T Caskey
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·I M BelyakovJ A Berzofsky
Aug 24, 1999·Gene Therapy·R StripeckeD Kohn
Mar 27, 2001·Blood·M RosenzweigR P Johnson
Dec 12, 2001·Journal of Virology·Valérie Molinier-FrenkelJean-Gérard Guillet
May 14, 2003·Circulation·L Maria BelalcazarLawrence Chan
Jul 12, 2003·The Journal of Biological Chemistry·Valérie Molinier-FrenkelJean-Gérard Guillet
Mar 3, 2004·Journal of Virology·Shawn M SumidaDan H Barouch
Aug 6, 2004·Gene Therapy·A HaruiS K Basak
Dec 29, 2004·Vaccine·Kent R Van KampenDe-chu C Tang
Feb 3, 2005·Human Gene Therapy·Daniel A Muruve
Oct 19, 2005·Gene Therapy·R AlbaM Chillon
Jan 4, 2006·The Journal of Gene Medicine·Chang-Xin ShiMary M Hitt
Jan 27, 2006·Gene Therapy·D R KoehlerJ Hu
Jan 28, 2006·Journal of Virology·Wentao GaoAndrea Gambotto
Aug 19, 2007·Human Gene Therapy·Can XieZhi Shang
Dec 21, 2007·Journal of Virology·Jae-Rang YuJun Chang
Feb 22, 2008·Gene Therapy·N Brunetti-Pierri, P Ng
Feb 24, 2009·Biochemical and Biophysical Research Communications·Yuanhui FuTao Hong
Apr 1, 2009·PloS One·Eric A WeaverMichael A Barry
Citations
Jun 8, 2013·Virology Journal·Yuan-Hui FuTao Hong
Jan 2, 2015·Vaccines·Shailbala SinghK Jagannadha Sastry
Dec 14, 2011·Journal of Reproductive Immunology·Rajesh K Naz
Mar 31, 2010·Biochemical and Biophysical Research Communications·Yuan-Hui FuTao Hong
Nov 19, 2011·The Journal of Gene Medicine·Matthias W KronFlorian Kreppel
Feb 25, 2014·Antiviral Research·Yuan-hui FuTao Hong
Oct 27, 2015·PloS One·Alexander Badamchi-ZadehRobin J Shattock
Aug 24, 2013·Expert Review of Respiratory Medicine·Patricia A JorqueraRalph A Tripp