Intranigral ventral mesencephalic grafts and nigrostriatal injections of glial cell line-derived neurotrophic factor restore dopamine release in the striatum of 6-hydroxydopamine-lesioned rats

Experimental Brain Research
F I TangY Wang

Abstract

We have previously reported that grafting of fetal ventral mesencephalic (VM) tissue to the nigral region of unilaterally 6-hydroxydopamine (6-OHDA)-lesioned rats, in conjunction with glial cell line-derived neurotrophic factor (GDNF) injection between nigra and striatum, restores nigrostriatal tyrosine hydroxylase (TH) immunoreactivity. In this study, we investigated the electrochemical indices of dopamine (DA) release in these grafted animals in the striatum and nigra. Adult Sprague-Dawley rats were anesthetized and unilaterally injected with 6-OHDA into the medial forebrain bundle. The completeness of lesions was tested by measuring methamphetamine-induced rotations. One to two months after 6-OHDA administration, fetal VM tissues were grafted in the lesioned nigral area followed by injection of GDNF, brain-derived neurotrophic factor (BDNF), or phosphate-buffered saline (PBS), along a tract from nigra to striatum. Animals receiving transplantation and GDNF, but not BDNF or PBS, injection showed a significant decrease in rotation 1-3 months after grafting. High-speed chronoamperometric recording techniques, using Nafion-coated carbon fiber electrodes, were used to evaluate DA overflow in the striatum. We found that 6-OHDA les...Continue Reading

Citations

Jul 12, 2011·Journal of Materials Science. Materials in Medicine·Elisa TamarizVíctor M Castaño
Mar 22, 2003·Journal of Neuroscience Methods·A E PeaireJohn W Commissiong
Sep 22, 2006·American Journal of Therapeutics·Sheng Chen, Weidong Le
Apr 6, 2005·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Yossef S LevyDaniel Offen
Nov 13, 2008·CNS Drugs·Fabio FumagalliMarco Andrea Riva
Jun 22, 2011·Cytokine & Growth Factor Reviews·Aideen M Sullivan, André Toulouse
Nov 20, 2008·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Zivar Salehi, Farhad Mashayekhi
Jan 11, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Feng ZhangJing-Shan Shi
Feb 5, 2002·Expert Opinion on Pharmacotherapy·C B Hurelbrink, R A Barker
Jul 11, 2014·Frontiers in Systems Neuroscience·Naoki TajiriCesar V Borlongan
Aug 12, 2005·Neurorehabilitation and Neural Repair·W-L Kuan, R A Barker
Mar 26, 2005·Cell Transplantation·Yung-Hsiao ChiangYun Wang
Dec 1, 2004·The Journal of Nutrition·Fu-In Tang, Ien-Lan Wei
May 26, 2017·Frontiers in Cell and Developmental Biology·Jea Y LeeSandra A Acosta

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