Intranuclear and higher-order chromatin organization of the major histone gene cluster in breast cancer.

Journal of Cellular Physiology
Andrew FritzG S Stein

Abstract

Alterations in nuclear morphology are common in cancer progression. However, the degree to which gross morphological abnormalities translate into compromised higher-order chromatin organization is poorly understood. To explore the functional links between gene expression and chromatin structure in breast cancer, we performed RNA-seq gene expression analysis on the basal breast cancer progression model based on human MCF10A cells. Positional gene enrichment identified the major histone gene cluster at chromosome 6p22 as one of the most significantly upregulated (and not amplified) clusters of genes from the normal-like MCF10A to premalignant MCF10AT1 and metastatic MCF10CA1a cells. This cluster is subdivided into three sub-clusters of histone genes that are organized into hierarchical topologically associating domains (TADs). Interestingly, the sub-clusters of histone genes are located at TAD boundaries and interact more frequently with each other than the regions in-between them, suggesting that the histone sub-clusters form an active chromatin hub. The anchor sites of loops within this hub are occupied by CTCF, a known chromatin organizer. These histone genes are transcribed and processed at a specific sub-nuclear microenviron...Continue Reading

References

Mar 23, 2001·Breast Cancer Research and Treatment·S J SantnerF R Miller
Jun 17, 2003·Nature Genetics·Jeffrey J RoixTom Misteli
Jul 25, 2003·Experimental Cell Research·Katherine L DunnJames R Davie
Jul 9, 2004·Genome Biology·Luis A ParadaTom Misteli
Sep 3, 2004·Nature Reviews. Cancer·Daniele ZinkJeffrey A Nickerson
May 29, 2007·Gene·Zbigniew Dominski, William F Marzluff
Aug 5, 2008·Biochimica Et Biophysica Acta·Stephan Kadauke, Gerd A Blobel
Aug 5, 2008·Cell Cycle·Lucilla Bongiorno-BorboneVincenzo De Laurenzi
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Sep 24, 2008·The Journal of Cell Biology·Jill M BrownVeronica J Buckle
Oct 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Prachi N GhuleGary S Stein
Nov 7, 2009·Diagnostic Cytopathology·Pranab Dey
Aug 4, 2010·Methods in Molecular Biology·Henriette O'GeenPeggy J Farnham
Dec 16, 2011·Current Opinion in Genetics & Development·Ivan Krivega, Ann Dean
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jun 2, 2012·Methods : a Companion to Methods in Enzymology·Jon-Matthew BeltonJob Dekker
Jun 23, 2012·Nature·Philip J StephensMichael R Stratton
Aug 7, 2012·Molecular Cell·Pedro P RochaJane A Skok
Sep 1, 2012·Nature Protocols·Jianxing FengXiaole Shirley Liu
Sep 8, 2012·Genome Research·Jennifer HarrowTim J Hubbard
Sep 25, 2012·Nature·UNKNOWN Cancer Genome Atlas Network
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Nov 13, 2012·Cell·E Josephine ClowneyStavros Lomvardas
Mar 30, 2013·Science·Mario L SuvàBradley E Bernstein
May 28, 2013·Molecular Cell·Jennifer E Phillips-Cremins, Victor G Corces
May 7, 2014·Molecular and Cellular Biology·Prachi N GhuleGary S Stein

❮ Previous
Next ❯

Citations

Apr 23, 2019·Journal of Cellular Physiology·Ian A KentTanmay P Lele
Mar 18, 2020·Journal of Cellular Physiology·Andrew J FritzGary S Stein
Jul 1, 2020·Journal of the Royal Society, Interface·Maria Rita FumagalliCaterina A M La Porta
Aug 26, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Paul J SmithRachel J Errington
Sep 28, 2018·The Journal of Cell Biology·Shweta MendirattaGenevieve Almouzni
Dec 7, 2020·Journal of Molecular Biology·Inma PonteAlicia Roque
Sep 13, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Chen-Yan ZhangSheng-Xiang Lin
Mar 21, 2021·Epigenetics & Chromatin·Beoung Hun Lee, Suhn K Rhie
Feb 24, 2018·Biochemistry·Dahyana Arias Escayola, Karla M Neugebauer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.