Intraoperative examination of sentinel lymph nodes by immunohistochemical staining in patients with breast cancer

European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology
I-K LeeY H Ryu

Abstract

To performed a prospective investigation of the relative merits of rapid cytokeratin immunohistochemical (CK-IHC) staining of the SLN removed during the operation of breast cancer patients. Between December 2002 and March 2004, 62 patients with T1 and T2 breast cancer were enrolled after undergoing successful sentinel lymph node biopsy. Eighty-nine sentinel lymph nodes (mean number, 1.44) were biopsied and first examined by hematoxylin-eosin (H&E) stained frozen section. All the tumour free sentinel lymph nodes by H&E stained frozen section were immunostained for cytokeratin using a rapid immunohistochemical assay (Cytokeratin (PAN), 1:50, Novocastra Lab., Newcastle, UK) during the operations. Rapid IHC staining revealed seven positive sentinel lymph nodes that were negative for metastasis by H&E staining. This study showed a sensitivity of 92.86%, a specificity of 100%, an accuracy of 98.9%, and a negative predictive value of 98.7%. The intraoperative examination of sentinel lymph nodes is an accurate and effective way of predicting the axillary lymph node status of patients with breast cancers.

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Citations

Jun 15, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Muhammad T IdreesLiang Cheng
Jul 18, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Anne Marie Bak JyllingJudith Jensen
May 21, 2008·The Breast : Official Journal of the European Society of Mastology·Marianne HolmHenrik Lavlund Flyger
May 30, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Saska BrajkovicMartin Am Gijs
Aug 3, 2017·Journal of Pathology and Translational Medicine·Ju Yeon PyoSoonWon Hong
Aug 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masahiko TsujimotoNariaki Matsuura

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