Intrapatient diversity of Achromobacter spp. involved in chronic colonization of Cystic Fibrosis airways

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Chloé DupontHélène Marchandin

Abstract

Achromobacter spp. are increasingly identified in Cystic Fibrosis (CF) patients and their ability to persistently colonize the CF respiratory tract (CFRT) suggests that Achromobacter species possess adaptive characteristics. We studied genome dynamics in 118 isolates recovered from 13 patients with Achromobacter chronic colonization (5-26 isolates per patient recovered over 13-61 months). Isolates were identified to species level by nrdA gene sequencing, subjected to Pulsed-Field Gel Electrophoresis (PFGE) and multiplex rep-PCR (MR-PCR), and rrs intragenomic diversity was studied by PCR-Temporal Temperature Gel Electrophoresis (TTGE). Intrapatient diversity was assessed: (i) from dynamics of XbaI and/or SpeI-based pulsotypes, (ii) from comparison of MR-PCR profiles, and (iii) by longitudinal analysis of rrs intragenomic diversity. Patients were chronically colonized by Achromobacter xylosoxidans (n=10), Achromobacter dolens (n=1) or Achromobacter insuavis (n=2). All strains displayed genomic diversification over time but A. insuavis showed higher pulsotype diversity compared to other species. Intragenomic rrs heterogeneity was found in strains from 6 of 13 patients and may be persistently observed. Achromobacter genome evolutio...Continue Reading

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Citations

Sep 29, 2015·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Amy CowardJane F Turton
Apr 7, 2017·Genome Biology and Evolution·Julie JeukensRoger C Levesque
Aug 25, 2015·Applied and Environmental Microbiology·Fatima AbdouchakourEstelle Jumas-Bilak
Oct 24, 2017·Frontiers in Microbiology·Chloé DupontHélène Marchandin
Mar 11, 2021·MBio·Marija DmitrijevaChristian von Mering
Apr 14, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T GarrigosUNKNOWN Collaborators

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