Abstract
The aim of this study was to assess whether high-density lipoprotein (HDL) labeled with superparamagnetic iron oxide nanoparticles (SPIOs) and quantum dots was able to detect atherosclerotic lesions in mice after intravenous and intraperitoneal injection by multimodal imaging. Nanoparticle-labeled HDLs (NP-HDLs) were characterized in vitro by dynamic light scattering and size exclusion chromatography with subsequent cholesterol and fluorescence measurements. For biodistribution and blood clearance studies, NP-HDL(SPIOs) radiolabeled with (59)Fe (NP-HDL(59Fe-SPIOs)) were injected intravenously or intraperitoneally into ApoE knockout mice (n=6), and radioactivity was measured using a gamma counter. NP-HDL accumulation within atherosclerotic plaques in vivo and ex vivo was estimated by MRI at 7 Tesla, ex vivo confocal fluorescence microscopy, x-ray fluorescence microscopy, and histological analysis (n=3). Statistical analyses were performed using a 2-tailed Student t-test. In vitro characterization of NP-HDL confirmed properties similar to endogenous HDL. Blood concentration time curves showed a biexponential decrease for the intravenous injection, whereas a slow increase followed by a steady state was noted for intraperitoneal in...Continue Reading
References
Jan 1, 1977·Physics in Medicine and Biology·J S BraunsfurthH C Heinrich
Jan 23, 1992·The New England Journal of Medicine·V FusterJ H Chesebro
Jul 1, 1991·Biochimica Et Biophysica Acta·T M AllenA Yau-Young
Jul 25, 1993·Biochimica Et Biophysica Acta·T M AllenL S Guo
Sep 23, 2000·Nature·A J Lusis
Dec 26, 2001·Journal of the American Chemical Society·T HyeonH B Na
May 15, 2002·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·A ShaishD Harats
Oct 15, 2003·Circulation·Morteza NaghaviJames T Willerson
Jun 9, 2004·Circulation·Marc SirolZahi A Fayad
Oct 8, 2004·Journal of the American Chemical Society·Dmitri V TalapinHorst Weller
Dec 17, 2004·Journal of the American Chemical Society·Juan C FriasZahi A Fayad
Mar 2, 2005·Nature Reviews. Drug Discovery·Patrick Linsel-Nitschke, Alan R Tall
Sep 27, 2006·Circulation·Matthias NahrendorfRalph Weissleder
Oct 13, 2006·Nano Letters·Juan C FriasEdward A Fisher
Jan 11, 2007·Surgical Neurology·Masaru HondaIzumi Nagata
Sep 13, 2007·Radiology·Rebecca KuhlpeterChristoph Bremer
Mar 14, 2008·Current Drug Targets·Jonathan E FeigEdward A Fisher
Jun 4, 2008·Current Opinion in Cardiology·Matilda FlorentinDimitri P Mikhailidis
Oct 23, 2008·Nano Letters·David P CormodeWillem J M Mulder
Mar 7, 2009·Nature Nanotechnology·Oliver T BrunsJoerg Heeren
Jul 30, 2010·Radiology·David P CormodeZahi A Fayad
Apr 8, 2011·Current Pharmaceutical Biotechnology·Joerg Heeren, Oliver Bruns
Oct 22, 2011·Nature Reviews. Drug Discovery·Mark E LobattoWillem J M Mulder
Jul 17, 2012·ACS Nano·Barbara FreundPeter Nielsen
Citations
Oct 3, 2014·Nanoscale Research Letters·Qinghua WuJunying Miao
Jul 2, 2015·Biochemical and Biophysical Research Communications·Maya JuenetDidier Letourneur
Oct 31, 2016·Advanced Drug Delivery Reviews·C Shad ThaxtonDavid P Cormode
Apr 28, 2017·Pulmonary Circulation·Nura A MohamedJane A Mitchell
Feb 11, 2016·Chemical Society Reviews·Neus FeliuWolfgang J Parak
Jun 29, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roxana Cristina PopescuDiana Savu
Jan 11, 2018·International Journal of Molecular Sciences·Binh T T PhamBrian S Hawkett
Aug 7, 2018·British Journal of Pharmacology·Joseph Pierre AboumsallemBart De Geest
Aug 5, 2017·PloS One·Caroline JungHarald Ittrich
Aug 19, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Akmal A El-GhorHanan Ramadan H Mohamed
Dec 18, 2015·Investigative Radiology·Caroline Sophie Laura JungHarald Ittrich
Nov 10, 2017·Nature Biomedical Engineering·Oliver T BrunsMoungi G Bawendi
Feb 6, 2020·Scientific Reports·Karolina StachnikAlke Meents
Jul 1, 2016·Chemical Science·Lyndsay M RandolphNathan C Gianneschi
Jan 28, 2020·Nano Convergence·Jun ChenHo-Wook Jun
Jun 21, 2020·Chemistry and Physics of Lipids·Liliana Aranda-LaraKeila Isaac-Olivé
Sep 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dennis Pedersbæk, Jens B Simonsen
Feb 7, 2020·Current Drug Delivery·Saleh A AlanaziGamaleldin I Harisa
Aug 15, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·John C Stendahl, Albert J Sinusas
Aug 22, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·John C Stendahl, Albert J Sinusas
Jul 25, 2021·International Journal of Environmental Research and Public Health·Elizabeth FiremanMordechai Reuven Kramer