Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice

Molecular Medicine Reports
Jia Lin SongDao Zhang Cai

Abstract

Vascular endothelial growth factor (VEGF) is expressed in articular cartilage and increases in expression levels have been associated with the progression of osteoarthritis (OA). Thalidomide is a drug that has been reported to inhibit angiogenesis and reduce VEGF production by downregulating VEGF expression. The objective of the present study was to determine whether intraperitoneal administration of thalidomide may attenuate early OA development in mice. Male C57BL/6 mice (10‑weeks‑old) were randomly assigned into the destabilization of the medial meniscus (Dmm) with thalidomide treatment (Dmm+Th), Dmm and Sham groups equally. An OA model was induced surgically in Dmm+Th and Dmm groups, and mice of the Dmm+Th group were subsequently treated with an intraperitoneal injection of thalidomide (200 mg/kg/day). At 2 and 4 weeks following surgery, the pathological alterations in cartilage samples were assessed qualitatively by hematoxylin and eosin staining and Safranin O/Fast green staining, and quantitatively by the Osteoarthritis Research Society International scoring system. The mRNA expression levels of matrix metalloproteinase‑13 (MMP‑13) and VEGF were measured by reverse transcription‑quantitative polymerase chain reaction. Th...Continue Reading

References

Jun 15, 1989·Biochemical and Biophysical Research Communications·N Ferrara, W J Henzel
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J D'AmatoJ Folkman
Feb 12, 1996·FEBS Letters·A J FosangP J Neame
Nov 24, 1999·The New England Journal of Medicine·S SinghalB Barlogie
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 8, 2002·Nature Reviews. Molecular Cell Biology·Constance E Brinckerhoff, Lynn M Matrisian
Jan 1, 2003·The American Journal of Pathology·Hiroyuki EnomotoYasunori Okada
Apr 17, 2003·Postgraduate Medical Journal·J N Gordon, P M Goggin
Mar 31, 2004·Radiologic Clinics of North America·David T Felson
Jun 4, 2004·The New England Journal of Medicine·Herbert HurwitzFairooz Kabbinavar
Jun 28, 2005·Osteoarthritis and Cartilage·Velandai K SrikanthGraeme Jones
Jul 20, 2005·Journal of Surgical Oncology·Jurstine DaruwallaChris Christophi
Aug 1, 2007·Nature Clinical Practice. Rheumatology·Dahlia Lainer-Carr, Ernest Brahn
Sep 6, 2008·Endoscopy·S F AlbertoJ de Deus
Jan 30, 2009·Arthritis and Rheumatism·Estibaliz LozaUNKNOWN Artrocad Study Group
May 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christina TherapontosNeil Vargesson
Jul 20, 2010·Amino Acids·T Klein, R Bischoff
Nov 22, 2011·Journal of Gastroenterology and Hepatology·Honghong TanShudong Xiao
Dec 6, 2011·International Journal of Cancer. Journal International Du Cancer·Niels F AndersenAnnette J Vangsted
Dec 20, 2011·Osteoarthritis and Cartilage·P M van der Kraan, W B van den Berg
Jul 16, 2014·Nature Reviews. Rheumatology·Flavia M Cicuttini, Anita E Wluka
Oct 15, 2014·Calcified Tissue International·Bingjiang XiaPeijian Tong
Oct 22, 2014·Methods in Molecular Biology·Jennifer H JonasonRegis J O'Keefe
Dec 18, 2014·BMC Musculoskeletal Disorders·Quan YuanChun-Hou An
Jan 7, 2015·Disease Models & Mechanisms·Sarah ThysenRik J U Lories
Mar 10, 2015·Lancet·S Glyn-JonesA J Carr
Jun 6, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Neil Vargesson
Jun 9, 2016·Arthritis Care & Research·Ilana N AckermanCaroline A Brand

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May 3, 2019·BioMed Research International·Elisa BelluzziVeronica Macchi

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