Intraspecific genetic diversity of lactic acid bacteria from malolactic fermentation of Cencibel wines as derived from combined analysis of RAPD-PCR and PFGE patterns

Food Microbiology
Patricia RuizM Llanos Palop

Abstract

Three molecular techniques, randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), pulsed field gel electrophoresis (PFGE) and differential display-polymerase chain reaction (DD-PCR) have been used to assess the intraspecific diversity of the lactic microbiota responsible for spontaneous malolactic fermentation (MLF) in Cencibel wines made at a cellar in Castilla-La Mancha (Spain). The results indicate that RAPD-PCR and PFGE are of value in typing this microbiota. Better discrimination was achieved by RAPD-PCR. Reproducibility using DD-PCR was not good, which makes this method unsuitable. Combined numerical analysis of the patterns obtained from RAPD-PCR and PFGE allowed a better discrimination; this would therefore be a suitable tool to discriminate the diversity of bacterial communities like those found in MLF of wines. Genetic diversity data from combined numerical analysis suggest that there is considerable microbial diversity within MLF of Cencibel wines, with some genotypes coinciding in the two vinifications analysed.

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Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·Fátima Pérez-MartínMaría Llanos Palop
Apr 3, 2013·World Journal of Microbiology & Biotechnology·Bárbara Mercedes Bravo-FerradaLiliana Semorile
Apr 11, 2012·TheScientificWorldJournal·Lucía González-ArenzanaIsabel López-Alfaro
Jun 16, 2014·Food Microbiology·Maria Stella CappelloGiacomo Zapparoli
May 1, 2012·International Journal of Food Microbiology·Pedro Miguel Izquierdo CañasMaría de los Llanos Palop Herreros
Jun 16, 2014·Food Microbiology·Fátima Pérez-MartínMaría Llanos Palop
Apr 6, 2013·International Journal of Food Microbiology·Fátima Pérez-MartínMaría Llanos Palop
Apr 1, 2014·Journal of Bioscience and Bioengineering·Lucía González-ArenzanaIsabel López-Alfaro
Jan 7, 2010·International Journal of Food Microbiology·Patricia RuizMaría Llanos Palop
Aug 12, 2009·Journal of Bioscience and Bioengineering·Pedro Miguel Izquierdo CañasMaria Llanos Palop Herreros
Aug 29, 2012·Journal of Food Science·Patricia RuizMaría Llanos Palop
Jan 20, 2015·Journal of Food Science·Luana Faria SilvaAna Lúcia Barretto Penna
Apr 8, 2015·Food Microbiology·Lucía González-ArenzanaIsabel López-Alfaro
Oct 22, 2016·International Journal of Food Microbiology·Patricia RuizMaría Llanos Palop
Jan 30, 2018·Journal of Applied Microbiology·M G BonomoG Salzano
Nov 24, 2016·Frontiers in Microbiology·Dalia E Miranda-CastillejaSofía M Arvizu-Medrano
Mar 2, 2018·Frontiers in Microbiology·Jaime RomeroCarla Jara
Apr 13, 2012·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Miroslava KačániováKatarína Rovná
May 14, 2009·Journal of Food Protection·P M Izquierdo CañasM L L Palop Herreros
Aug 24, 2017·FEMS Microbiology Reviews·Eveline J Bartowsky
Dec 4, 2020·Animals : an Open Access Journal From MDPI·Álvaro Rafael QuintanaRamón Arias

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