PMID: 8582706Nov 1, 1995Paper

Intrathecal infusion of brain-derived neurotrophic factor protects nigral dopaminergic neurons from degenerative changes in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced monkey parkinsonian model

[Hokkaido igaku zasshi] The Hokkaido journal of medical science
M Takeda

Abstract

Protective effect of BDNF on nigrostriatal dopaminergic neurons was evaluated with MPTP-induced parkinsonian model in monkey. Twelve adult female Japanese monkeys weighing about 7kg were lesioned with systemic infusion of MPTP according to the following dosing schedules. Group I: normal control (n = 2), group II: continuous infusion of 15mg of MPTP with osmotic minipump (Alzet: 2ML2, mean pumping rate 5 microliters/hour) for 14 days (n = 2), group III: bolus injections of 5mg of MPTP on day 0, 4 and 7 (n = 2), group IV: bolus injections of 5mg of MPTP on day 0 and 4 (n = 6). Half of the animals in each groups except groupI were treated with BDNF, and the other half served as control. In BDNF-treated animals, total 6.5 ml of conditioned culture medium of Chinese hamster ovary containing 1 micrograms protein/ml of BDNF was administered into cisterna magna by osmotic minipump (Alzet: 2ML2) implanted on day 0. Culture medium without BDNF activity was also administered to control animals in the same manner. Neurological findings were evaluated with the Monkey Parkinsonism Rating Scale (MPRS) by Kurlan et al. The animals were sacrificed on day 14 and histological findings of the pars compacta was investigated. All the animals in grou...Continue Reading

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