Intrathecally produced IgG and IgM antibodies to recombinant VlsE, VlsE peptide, recombinant OspC and whole cell extracts in the diagnosis of Lyme neuroborreliosis

Medical Microbiology and Immunology
Gerold StanekF Strle

Abstract

Detection of intrathecally produced specific antibodies (AI) is essential in the diagnosis of Lyme neuroborreliosis (LNB); however, the performance of various newer AI detection methods has not been systematically assessed. Here we assessed and compared advanced test systems for detecting borrelia IgG-AI and IgM-AI. Serum and cerebrospinal fluid (CSF) samples from well-defined LNB and tick-borne encephalitis (TBE) patients, 25 each, were tested with three antibody detection systems, one based on chemiluminescence (CLA) and two based on enzyme-linked immunosorbent assays (ELISA), employing different antigens for detection of IgG and IgM antibodies. In samples from patients with LNB, IgG-AI was detected in 20 samples by CLA, 19 by ELISA1, and 22 by ELISA2, and IgM-AI was detected in 16 samples by CLA, six by ELISA1, and 11 by ELISA2. In samples from TBE patients, IgG-AI was positive in one case by CLA and ELISA2, and in 7 cases by ELISA1, whereas IgM-AI was positive in one case by CLA and in none by ELISA. IgG-AI and IgM-AI were not detected within the first week of disease. Duration of disease correlated with IgG-AI while IgM-AI results were heterogeneous for each test assay. Moreover, the levels of IgG-AI, but not IgM-AI, corre...Continue Reading

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Sep 10, 2011·Lancet·Gerold StanekFranc Strle

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Citations

May 11, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John J Halperin
Dec 3, 2014·Journal of Autoimmunity·Andrea T BorchersM Eric Gershwin
Jun 13, 2018·FEMS Microbiology Reviews·Gerold Stanek, Franc Strle
Oct 16, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Heidi SillanpääPekka Lahdenne

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