PMID: 9653164Jul 8, 1998Paper

Intrathymic selection of NK1.1(+)alpha/beta T cell antigen receptor (TCR)+ cells in transgenic mice bearing TCR specific for chicken ovalbumin and restricted to I-Ad

Proceedings of the National Academy of Sciences of the United States of America
C IwabuchiK Onoé

Abstract

Generation and negative selection of NK1.1(+)alpha/beta T cell receptor (TCR)+ thymocytes were analyzed using TCR-transgenic (B10. D2 x DO10)F1 and (C57BL/6 x DO10)F1 mice and Rag-1(-/-)/DO10 mice, which had been established by breeding and backcrossing between Rag-1(-/-) and DO10 mice. Almost all T cells from these mice were shown to bear Valpha13/Vbeta8.2 that is specific for chicken ovalbumin (cOVA) and restricted to I-Ad. A normal proportion of the NK1.1(+) Valpha13/Vbeta8.2(+) thymocytes was generated in these mice. However, the actual cell number of both NK1.1(+) and NK1.1(-) thymocytes in I-Ad/d mice (positive selecting background) was larger than that in I-Ab/d mice (negative selecting background). Markedly low but significant proportions of NK1.1(+) Valpha13/Vbeta8.2(+) cells were detected in the spleens from I-Ad/d and I-Ab/d mice. It was shown that the splenic NK1.1(+) T cells of the I-Ab/d mice were anergized against stimulation through TCR. When (B10.D2 x DO10)F1 and (C57BL/6 x DO10)F1 mice were given cOVA, extensive or intermediate elimination of NK1.1(+)alpha/betaTCR+ thymocytes was induced in I-Ad/d or I-Ab/d mice, respectively. However, the clonal elimination was not as complete as that seen in the major NK1.1(...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H AraseK Onoé
Nov 1, 1991·The Journal of Experimental Medicine·H von BoehmerB Rocha
Dec 1, 1995·The Journal of Experimental Medicine·A Bendelac
May 12, 1995·Science·A BendelacR R Brutkiewicz
Jun 1, 1995·Current Opinion in Immunology·A Bendelac
Sep 1, 1995·The Journal of Experimental Medicine·H R MacDonald
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y AdachiM Taniguchi
Apr 1, 1994·The Journal of Experimental Medicine·T Yoshimoto, W E Paul
Jul 1, 1994·The Journal of Experimental Medicine·M C Coles, D H Raulet
Mar 1, 1996·The Journal of Experimental Medicine·T Ohteki, H R MacDonald
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·M TaniguchiY Makino
Oct 1, 1996·The Journal of Experimental Medicine·A BendelacO Lantz
Nov 1, 1996·The Journal of Experimental Medicine·C P LiuP Marrack
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·K NakagawaK Onoé

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Citations

Oct 6, 2007·Immunologic Research·Kazunori OnoéKazuya Iwabuchi
Nov 30, 2000·Immunology Today·D I GodfreyA G Baxter
Mar 25, 2004·Nature Reviews. Immunology·Dale I GodfreyLuc Van Kaer
May 20, 2003·Scandinavian Journal of Immunology·S KeshavarziM Cascalho
Mar 1, 2006·European Journal of Immunology·Gerhard WingenderPercy A Knolle
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Izumi KawachiSusan Gilfillan
May 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stephan D GadolaVincenzo Cerundolo
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K NambaK Onoé
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christophe Viret, Charles A Janeway

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