Intratracheal aerosolization of viral vectors to newborn pig airways.

BioTechniques
Ashley L Cooney, Patrick L Sinn

Abstract

Gene therapy for airway diseases requires efficient delivery of nucleic acids to the airways. In small animal models, gene delivery reagents are commonly delivered as a bolus dose. However, large animal models are often more relevant for the transition from preclinical studies to human trials. Aerosolizing viral vectors to the lungs of large animals can maximize anatomical distribution. Here, we describe a technique for aerosolization of viral vectors to the airways of newborn pigs. Briefly, a pig is anesthetized and intubated with an endotracheal tube, and a microsprayer is passed through the endotracheal tube. A fine mist is then sprayed into the distal trachea. Widespread and uniform distribution of transgene expression is critical for developing successful lung gene therapy treatments.

References

Aug 3, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·D R KoehlerJ Hu
Feb 8, 2005·American Journal of Respiratory Cell and Molecular Biology·Patrick L SinnPaul B McCray
Jan 27, 2006·Gene Therapy·D R KoehlerJ Hu
Feb 25, 2006·American Journal of Respiratory Cell and Molecular Biology·Heather DavidsonDeborah Penque
Jan 20, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gerry McLachlanD David S Collie
Jul 8, 2009·Journal of Liposome Research·Maria Manuela GasparCarsten Ehrhardt
Dec 30, 2009·The Journal of Clinical Investigation·Andrew A WilsonDarrell N Kotton
Oct 22, 2010·Gene Therapy·U GriesenbachE W F W Alton
Mar 1, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mayumi OaklandPaul B McCray
Nov 29, 2012·Molecular Therapy. Nucleic Acids·Patrick L SinnPaul B McCray
Sep 23, 2016·JCI Insight·Ashley L CooneyPaul B McCray
Jul 12, 2018·Human Gene Therapy Methods·Chantelle McIntyreDavid Parsons
Nov 9, 2018·Genes·Ashley L CooneyPatrick L Sinn
Dec 13, 2018·Frontiers in Pharmacology·Martin Donnelley, David W Parsons
Mar 22, 2019·Molecular Therapy. Nucleic Acids·Ignacio CaballeroGeorges Vassaux
Jun 12, 2019·American Journal of Respiratory Cell and Molecular Biology·Ashley L CooneyPatrick L Sinn

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Methods Mentioned

BETA
PCR
sedation
dissection
x-ray imaging
fluorescence microscopy
dissecting

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