Intratumor heterogeneity of biomarker expression in breast carcinomas

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
D C ChhiengS Beeken

Abstract

Small biopsy samples are used increasingly to assess the biomarker expression for prognostic information and for monitoring therapeutic responses prior to and during neoadjuvant therapy. The issue of intratumor heterogeneity of expression of biomarkers, however, has raised questions about the validity of the assessment of biomarker expression based on limited tissue samples. We examined immunohistochemically the expression of HER-2neu (p185erbB-2), epidermal growth factor receptor (EGFR), Bcl-2, p53, and proliferating cell nuclear antigen (PCNA) in 30 breast carcinomas using archived, paraffin embedded tissue and determined the extent of intratumor heterogeneity. Each section was divided into four randomly oriented discrete regions, each containing a portion of the infiltrating carcinoma. For each tumor, the entire lesion and four regions were analyzed for the expression of these markers. Scores of both membrane and cytoplasmic staining of HER-2neu and EGFR, scores of cytoplasmic staining of Bcl-2, and scores of nuclear staining of both p53 and PCNA were recorded. The intensity of staining and the proportion of immunostained cells were determined. A semiquantitative immunoscore was calculated by determining the sum of the produ...Continue Reading

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Citations

Mar 29, 2014·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·R W Dapson
Jan 19, 2012·The Journal of Molecular Diagnostics : JMD·Sian M DibbenRichard D Kennedy
Dec 26, 2017·Frontiers in Medicine·Gulisa Turashvili, Edi Brogi
Oct 17, 2008·Molecular Cancer Research : MCR·Joanna M IlvesaroKatri S Selander
May 21, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Aziza NassarCynthia Cohen

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