Intratumoural budding (ITB) in preoperative biopsies predicts the presence of lymph node and distant metastases in colon and rectal cancer patients

British Journal of Cancer
Inti ZlobecAlessandro Lugli

Abstract

In colorectal cancer (CRC), tumour budding at the invasion front is associated with lymph node (LN) and distant metastasis. Interestingly, tumour budding can also be detected in biopsies (intratumoural budding; ITB) and may have similar clinical importance. Here we investigate whether ITB in preoperative CRC biopsies can be translated into daily diagnostic practice. Preoperative biopsies from 133 CRC patients (no neoadjuvant therapy) underwent immunohistochemistry for pan-cytokeratin marker AE1/AE3. Across all biopsies for each patient, the densest region of buds at × 40 (high-power field; HPF) was identified and buds were counted. A greater number of tumour buds in the biopsy was associated with pT stage (P=0.0143), LN metastasis (P=0.0007), lymphatic (P=0.0065) and venous vessel invasion (P=0.0318) and distant metastasis (cM1) (P=0.0013). Using logistic regression, a 'scale' was developed to estimate the probability of LN and distant metastasis using the number of tumour buds (e.g. 10 buds per HPF: 64% chance of LN metastasis; 30 buds per HPF: 86% chance). Inter-observer agreement for ITB was excellent (intraclass correlation coefficient: 0.813). Tumour budding can be assessed in the preoperative biopsy of CRC patients. It is...Continue Reading

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Citations

Feb 24, 2015·Virchows Archiv : an International Journal of Pathology·Viktor H KoelzerAlessandro Lugli
Mar 26, 2015·Frontiers in Medicine·Heather Dawson, Alessandro Lugli
Sep 2, 2014·Frontiers in Oncology·Viktor H KoelzerAlessandro Lugli
May 3, 2016·Journal of Clinical Medicine·Alexandru Dan GrigoreHerbert Levine
Nov 3, 2016·Therapeutic Advances in Gastroenterology·Marina De RosaPaolo Delrio
Mar 10, 2017·Scientific Reports·Tarig MagdeldinUmber Cheema
May 27, 2017·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Alessandro LugliPhil Quirke
Mar 2, 2018·Histopathology·Eva KaramitopoulouAlessandro Lugli
May 15, 2018·British Journal of Cancer·Philipp LohneisAnja Jühling
Feb 7, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Kyra B Berg, David F Schaeffer
Jul 25, 2018·Archives of Pathology & Laboratory Medicine·Soo-Jin Cho, Sanjay Kakar
Mar 18, 2015·Breast Cancer Research and Treatment·Bodour SalhiaCoya Tapia
Jun 11, 2019·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Hideki UenoUNKNOWN SACURA Study Group
Jul 25, 2019·The American Journal of Surgical Pathology·Mariko TanakaMasashi Fukayama
Sep 29, 2019·Histopathology·Heather DawsonAlessandro Lugli
Jul 18, 2015·Histopathology·Alhadi AlmangushIlmo Leivo
Nov 2, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Patricia AlamoRamón Mangues
Sep 10, 2020·Nature Reviews. Clinical Oncology·Alessandro LugliIris D Nagtegaal
Nov 29, 2015·Virchows Archiv : an International Journal of Pathology·Linde De SmedtXavier Sagaert
Dec 6, 2019·Frontiers in Oncology·Valeria MaffeisRocco Cappellesso
Dec 16, 2019·International Journal of Colorectal Disease·Andreas H MarxTill S Clauditz
Jan 30, 2020·Revista da Associação Médica Brasileira·Songul Peltek OzerMustafa Sit
Jul 4, 2020·Pathology Oncology Research : POR·Gorana AralicaPaško Konjevoda
Dec 21, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Leonardo S Lino-SilvaPatricia López-Correa
Dec 20, 2019·Journal of Medical Systems·Mohammad F A FauziMetin N Gurcan
Jul 1, 2020·British Journal of Cancer·Inti ZlobecAlessandro Lugli
Jan 7, 2021·Virchows Archiv : an International Journal of Pathology·Kazuki KawamuraKeiichi Ito
Apr 13, 2021·Virchows Archiv : an International Journal of Pathology·Inti ZlobecAlessandro Lugli
Jul 7, 2021·Annals of Diagnostic Pathology·Nazlı Sena SekerMustafa Fuat Acikalin
Aug 10, 2021·Applied Immunohistochemistry & Molecular Morphology : AIMM·Xiaoyun WenJela Bandovic

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Methods Mentioned

BETA
biopsy
to
biopsies
surgical resection
surgical
surgical resections

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