Intrauterine Transmission of Anaplasma phagocytophilum in Persistently Infected Lambs

Veterinary Sciences
Snorre StuenAnne Mette Sagen

Abstract

Anaplasma phagocytophilum, which causes the disease tick-borne fever (TBF), is the most important tick-borne pathogen in European animals. TBF may contribute to severe welfare challenges and economic losses in the Norwegian sheep industry. The bacterium causes a persistent infection in sheep and several other animal species. The objective of this study was to investigate whether intrauterine transmission occurs in persistently infected sheep. The study included thirteen 5-6-month-old unmated ewes, of which twelve were experimentally infected withA. phagocytophilum(GenBank acc. no. M73220). Four to six weeks later, all ewes were mated, and nine became pregnant. Blood samples were collected from these ewes and their offspring. If the lamb died, tissue samples were collected. The samples were analyzed with real-time PCR (qPCR) targeting themsp2gene. PCR-positive samples were further analyzed by semi-nested PCR and 16S rDNA sequencing. A total of 20 lambs were born, of which six died within two days. Six newborn lambs (30%) were PCR-positive (qPCR), of which one was verified by 16S rDNA sequencing. The present study indicates that intrauterine transmission ofA. phagocytophilumin persistently infected sheep may occur. The importance...Continue Reading

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Citations

Apr 2, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Vera RarNina Tikunova

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Methods Mentioned

BETA
PCR
electrophoresis
antibody assay

Software Mentioned

BLAST ( Basic Local Alignment Tool )

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