Intravaginal HPV DNA vaccination with electroporation induces local CD8+ T-cell immune responses and antitumor effects against cervicovaginal tumors

Gene Therapy
Y SunTc Wu

Abstract

Therapeutic human papillomavirus (HPV) vaccines have the potential to inhibit the progression of an established HPV infection to precancer and cancer lesions by targeting HPV oncoproteins. We have previously developed a therapeutic DNA vaccine encoding calreticulin (CRT) linked to E7, CRT/E7 DNA vaccine, for use in the treatment of HPV-associated lesions. Since the transfection efficiency of DNA vaccines administered in vivo is typically low, we examined the use of electroporation as well as different routes of administration to enhance antigen-specific tumor control. We tested the effects of the CRT/E7 DNA vaccine administered intramuscularly or intravaginally, with or without electroporation, on the generation of CD8+ T-cell immunity and therapeutic antitumor effects in HPV16 E7-expressing cervicovaginal tumor-bearing mice. We found that intravaginal vaccination of CRT/E7 DNA followed by electroporation-induced potent E7-specific CD8(+) T-cell responses in the cervicovaginal tract, compared with intramuscular injection followed by electroporation. Furthermore, tumor-bearing mice vaccinated intravaginally followed by electroporation had an enhanced survival, antitumor effects and local production of IFN-γ+CD8+ T cells compared...Continue Reading

References

Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
Jun 5, 2002·Nature Reviews. Cancer·Harald zur Hausen
Oct 29, 2009·Current Molecular Medicine·G GorfuK Ley
Jan 13, 2010·Immunologic Research·Ken LinT-C Wu
Feb 4, 2010·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Ken LinT C Wu
Jul 17, 2010·International Journal of Cancer. Journal International Du Cancer·Loane DecrausazDenise Nardelli-Haefliger
Nov 3, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cornelia L TrimbleAchim A Jungbluth
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Nov 13, 2012·The Journal of Clinical Investigation·Nicolas ÇuburuJohn T Schiller
Feb 15, 2013·Science Translational Medicine·Federico SandovalEric Tartour
Sep 7, 2013·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Nam Phuong TranT-C Wu
Dec 5, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chao-Yi WuT-C Wu
Jun 5, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ruey-Shyang SoongChien-Fu Hung

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Citations

Sep 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Todd BartkowiakMichael A Curran
Feb 24, 2016·Expert Review of Vaccines·Andrew YangChien-Fu Hung
Oct 13, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ashim K MitraDhananjay Pal
Jan 11, 2018·Human Gene Therapy·Max A ChengT-C Wu
Dec 7, 2017·Oncoimmunology·Stefano PieriniLorenzo Galluzzi
Mar 1, 2020·Nature Communications·Tae Heung KangChien-Fu Hung
May 28, 2020·Cancers·Ángela ÁyenHouria Boulaiz
Apr 20, 2019·Frontiers in Cellular and Infection Microbiology·Zelalem A MekonnenDanushka K Wijesundara

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Methods Mentioned

BETA
transfection
flow cytometry
FACS

Clinical Trials Mentioned

NCT00988559
NCT01493154
NCT01304524
NCT01634504

Software Mentioned

FlowJo
TriGrid

Related Concepts

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