Intravenous administration of 2,7,8-trimethyl-2-(beta-carboxyethyl)-6-hydroxy chroman (gamma-CEHC) to rats and determination of its plasma concentration and urinary sodium excretion

Biomedical Chromatography : BMC
Maiko TanabeK Imai

Abstract

A natriuretic hormone, 2,7,8-trimethyl-2-(beta-carboxyethyl)-6-hydroxy chroman (gamma-CEHC) was administered intravenously to male Sprague-Dawley rats and the plasma concentration of gamma-CEHC along with urinary sodium (Na+) excretion was investigated. The plasma gamma-CEHC concentrations were fluorimetrically determined by a column-switching HPLC method consisting of both phenyl and octadecyl silica columns, following a pre-column fluorescence derivatization with a fluorescence reagent, 4-N,N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ). In rats fed with a high-NaCl (8.0%) diet, plasma gamma-CEHC concentrations rapidly decreased by 20% in 15-45 min after the administration of gamma-CEHC, while Na+ excretion gradually increased with time. Considering these results, the Na+ excretion effect appeared not to be associated with plasma gamma-CEHC concentration. In addition, attempts were made to examine a main urinary metabolite of gamma-CEHC, a large amount of 6-O-sulfated gamma-CEHC found to be present in the urine using an HPLC-tandem mass spectrometry. Thus, it is plausible that gamma-CEHC was easily metabolized to 6-O-sulfated metabolite and excreted into urine in rats.

References

Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·W J WechterW H Wang
Mar 10, 2000·Biochemical and Biophysical Research Communications·R S Parker, J E Swanson
Apr 25, 2001·Kidney International·M A Vieira-CoelhoP Soares-da-Silva
Jul 7, 2001·Free Radical Biology & Medicine·M BirringerR Brigelius-Flohe
Apr 5, 2002·Biomedical Chromatography : BMC·Nozomi AoyamaKazuhiro Imai
Oct 9, 2002·The Journal of Nutrition·Marc BirringerRegina Brigelius-Flohé
May 6, 2003·Journal of Lipid Research·Hisako SaitoOsamu Igarashi

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Citations

Jul 13, 2005·Journal of Plant Physiology·Regina Brigelius-Flohé
Mar 17, 2016·World Journal of Biological Chemistry·Lisa SchmölzMaria Wallert
Dec 1, 2006·Nutrition Research Reviews·Regina Brigelius-Flohé

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