Intravenous immunoglobulin inhibits anti-glycoprotein IIb-induced platelet apoptosis in a murine model of immune thrombocytopenia

British Journal of Haematology
Valery LeytinJohn Freedman

Abstract

We have previously shown that injection of anti-glycoprotein (GP) IIb induces murine immune thrombocytopenia (ITP) and that intravenous immunoglobulin (IVIg) ameliorates ITP. We hypothesise that murine ITP may be associated with platelet apoptosis, which is upregulated by anti-GPIIb and downregulated by IVIg. The current study demonstrated that anti-GPIIb injection induced three critical apoptosis manifestations in platelets: (i) mitochondrial inner transmembrane potential (delta psi m) depolarisation; (ii) caspase-3 activation; and (iii) phosphatidylserine (PS) exposure. IVIg administration inhibited caspase-3 activation and PS exposure, but not delta psi m-depolarisation, in anti-GPIIb-treated platelets, demonstrating that IVIg ameliorates thrombocytopenia concomitantly with inhibiting late, but not early mechanisms of platelet apoptosis.

References

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May 3, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Valery Leytin, John Freedman
Jun 29, 2004·Biochemical and Biophysical Research Communications·Valery LeytinJohn Freedman

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Citations

Jun 6, 2009·Annals of Hematology·Paula Yurie TanakaJuliana Pereira
Mar 26, 2010·Annals of Hematology·John W Semple
Apr 23, 2010·Annals of Hematology·Oliver Speer, Markus Schmugge
Jul 4, 2012·Apoptosis : an International Journal on Programmed Cell Death·Syeda T TowhidFlorian Lang
Mar 3, 2012·Journal of Thrombosis and Thrombolysis·Armen V GyulkhandanyanValery Leytin
Mar 19, 2014·La Presse médicale·Douglas B CinesJohn W Semple
Jan 17, 2016·Environmental Toxicology and Pharmacology·Qing ZhaoKesheng Dai
Nov 8, 2011·Blood Reviews·Valery Leytin
Jul 31, 2008·Journal of Thrombosis and Haemostasis : JTH·J J LopezJ A Rosado
Jun 15, 2007·Journal of Thrombosis and Haemostasis : JTH·J J LopezJ A Pariente
Aug 14, 2013·British Journal of Haematology·Christopher G J McKenzieJohn W Semple
May 17, 2013·Seminars in Hematology·Stephan von GuntenHans-Uwe Simon
Oct 3, 2012·International Immunopharmacology·Lynnae M SmithDavid A Loeffler
Feb 18, 2017·Journal of Clinical Medicine·Anne ZuffereyJohn W Semple

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