Intravenous injection of allogeneic umbilical cord-derived multipotent mesenchymal stromal cells reduces the infarct area and ameliorates cardiac function in a porcine model of acute myocardial infarction

Stem Cell Research & Therapy
Meikuang LimZhongchao Han

Abstract

Multipotent mesenchymal stromal cell (MSC) therapy has been widely recognized as a feasible strategy for regenerating injured myocardial tissue. However, little is known about the efficacy of intravenous injection of allogeneic umbilical cord (UC) MSCs in preclinical models of porcine myocardial infarction. Different dosages of allogeneic UC-MSCs or the vehicle [phosphate-buffered saline (PBS)] were delivered intravenously into an acute myocardial infarction (AMI) porcine model twice after coronary ligation. Echocardiography was performed to examine the cardiac function and single photon emission computed tomography (SPECT) and positron emission tomography (PET)/computed tomography (CT) was performed to detect cardiac perfusion and nonviable myocardium. At the end of the experiment, 2,3,5-triphenyl-tetrazolium chloride (TTC) staining and Masson T staining were performed to determine the infarct area. The protein and gene expression levels associated with cardiac function, inflammation, and angiogenesis were examined by Western blot and real time polymerase chain reaction (PCR). In vivo trafficking of intravenous injection of allogeneic UC-MSCs enhanced green fluorescent protein (eGFP) was detected by real time PCR and immunoflu...Continue Reading

References

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Citations

Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Edoardo MaghinSveva Bollini
Jul 11, 2019·Future Medicinal Chemistry·Xiangnan ZhaoZongjin Li
May 3, 2019·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Annika HessJames T Thackeray
Feb 14, 2021·In Vitro Cellular & Developmental Biology. Animal·Haihua GengHongzhuan Sheng
Dec 31, 2020·International Journal of Molecular Sciences·Yi-Hsien FangYen-Wen Liu
Apr 20, 2021·Cardiovascular Research·Roberto BolliArunpreet Kahlon
May 18, 2021·Frontiers in Bioengineering and Biotechnology·Hye Sook ShinYasuhiro Shudo
May 18, 2021·Journal of Molecular Histology·Wei HuangAnying Li
Jun 13, 2020·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nina KosaricGeoffrey C Gurtner
Nov 17, 2021·Molecular and Cellular Biochemistry·Xian-Liang TangRoberto Bolli

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Methods Mentioned

BETA
transfection
flow cytometry
PCR
protein assay
electrophoresis
confocal microscopy

Software Mentioned

Xeleris workstation
SPSS
Image J
Myovation
OSEM
Xeleris

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