Intravenous injection of umbilical cord-derived mesenchymal stromal cells attenuates reactive gliosis and hypomyelination in a neonatal intraventricular hemorrhage model

Neuroscience
Takeo MukaiTokiko Nagamura-Inoue

Abstract

Intraventricular hemorrhage (IVH) is a frequent complication of preterm newborns, resulting in cerebral palsy and cognitive handicap as well as hypoxic ischemic encephalopathy and periventricular leukomalacia. In this study, we investigated the restorative effect on neonatal IVH by umbilical cord-derived mesenchymal stromal cells (UC-MSCs) cultured in serum-free medium (RM medium) for clinical application. UC-MSCs were cultured with αMEM medium supplemented with FBS or RM. A neonatal IVH mouse model at postnatal day 5 was generated by intraventricular injection of autologous blood, and mice were intravenously administered 1×105 UC-MSCs two days after IVH. Brain magnetic resonance imaging was performed at postnatal day 15, 22 and neurological behavioral measurements were performed at postnatal day 23, accompanied by histopathological analysis and cytokine bead assays in serum after IVH with or without UC-MSCs. Both UC-MSCs cultured with αMEM and RM met the criteria of MSCs and improved behavioral outcome of IVH mice. Moreover the RM group exhibited significant behavioral improvement compared to the control group. Histopathological analysis revealed UC-MSCs cultured with RM significantly attenuated periventricular reactive gliosi...Continue Reading

Citations

Jun 1, 2020·Stem Cells Translational Medicine·Beatriz Fernández-MuñozRosario Sanchez-Pernaute
Sep 23, 2020·American Journal of Perinatology·Tania Siahanidou, Christina Spiliopoulou
Apr 6, 2019·Frontiers in Physiology·Frank van BelFloris Groenendaal
Feb 13, 2020·F1000Research·Samata Singhi, Michael Johnston
Apr 4, 2019·Molecular Neurobiology·Eliana Baez-JuradoGeorge E Barreto
Mar 7, 2020·Frontiers in Cell and Developmental Biology·Anita C TruttmannJulien Puyal
Nov 12, 2020·International Journal of Molecular Sciences·Isabel Atienza-NavarroMonica Garcia-Alloza
Apr 4, 2021·International Journal of Molecular Sciences·Inês SerrenhoGraça Baltazar

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