Intravesical resiniferatoxin decreases spinal c-fos expression and increases bladder volume to reflex micturition in rats with chronic inflamed urinary bladders

BJU International
Paulo DinisFrancisco Cruz

Abstract

To evaluate the effect of intravesical resiniferatoxin on spinal c-fos expression and bladder volume at reflex micturition in rats with chronic urinary bladder inflammation. Of three groups of female Wistar rats, group 1 received cyclophosphamide (75 mg/kg body weight) intraperitoneally every third day (cyclophosphamide is an antitumoral agent that induces bladder inflammation after urinary excretion of its metabolite, acrolein); group 2 comprised sham-inflamed rats that received saline instead of cyclophosphamide, and group 3 received cyclophosphamide, as group 1, every third day but plus 10 nmol/L resiniferatoxin intravesically, through a urethral catheter, at 7 days. At 8 days, under urethane anaesthesia, a needle was inserted in the bladder dome and saline infused at 6 mL/h for 2 h. Finally the animals were perfusion-fixed through the ascending aorta with 4% paraformaldehyde. Transverse sections cut from L6 spinal cord segments were immunoreacted for Fos protein and positive cells in the dorsal horn counted. In a further set of equal groups the bladders were prepared in the same way under urethane anaesthesia and after 30-min of stabilization, saline was infused at 6 mL/h and the volume evoking reflex micturition determined...Continue Reading

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Citations

Oct 20, 2012·International Urogynecology Journal·Evangelia BakaliDouglas G Tincello
May 25, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·António Avelino, Francisco Cruz
Apr 1, 2009·The Clinical Journal of Pain·Omero Benedicto Poli-NetoAntonio Alberto Nogueira
Jun 3, 2014·Journal of Biomedical Science·Young-Sam ChoKhae-Hawn Kim
Jun 11, 2011·Neurourology and Urodynamics·Dale E BjorlingWade Bushman
Oct 10, 2014·British Journal of Pharmacology·A CoelhoL A Birder
Aug 6, 2015·Canadian Journal of Physiology and Pharmacology·Kajetan Juszczak, Piotr Maciukiewicz
Nov 18, 2016·Pharmaceuticals·Aaron D MickleDurga P Mohapatra
Aug 21, 2007·Neurourology and Urodynamics·Francisco Cruz, Paulo Dinis
Jan 24, 2007·Physiological Reviews·Bernd NiliusJohn A Peters
Nov 29, 2013·British Journal of Pharmacology·J Sousa-ValenteI Nagy
Feb 6, 2014·The European Journal of Neuroscience·João Sousa-ValenteIstvan Nagy
Oct 18, 2014·Molecular Medicine Reports·Young-Sam ChoKhae-Hawn Kim
Jan 11, 2018·Scientific Reports·Luke GrundyRuss Chess-Williams
Jul 20, 2017·Medicina·Łukasz DobrekPiotr Thor
Feb 5, 2021·Nature Reviews. Urology·Matthias VannesteWouter Everaerts

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