Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events

Nature Communications
Naoki HonkuraLena Claesson-Welsh

Abstract

The vasculature undergoes changes in diameter, permeability and blood flow in response to specific stimuli. The dynamics and interdependence of these responses in different vessels are largely unknown. Here we report a non-invasive technique to study dynamic events in different vessel categories by multi-photon microscopy and an image analysis tool, RVDM (relative velocity, direction, and morphology) allowing the identification of vessel categories by their red blood cell (RBC) parameters. Moreover, Claudin5 promoter-driven green fluorescent protein (GFP) expression is used to distinguish capillary subtypes. Intradermal injection of vascular endothelial growth factor A (VEGFA) is shown to induce leakage of circulating dextran, with vessel-type-dependent kinetics, from capillaries and venules devoid of GFP expression. VEGFA-induced leakage in capillaries coincides with vessel dilation and reduced flow velocity. Thus, intravital imaging of non-invasive stimulation combined with RVDM analysis allows for recording and quantification of very rapid events in the vasculature.

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Citations

Jul 10, 2019·Growth Factors·Zoe L Grant, Leigh Coultas
Jan 1, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Anouchka CosteDavid Entenberg
Aug 1, 2020·Frontiers in Physiology·Xiujuan LiLena Claesson-Welsh
Nov 28, 2020·Nature Communications·Jiang Lan FanNa Ji
Dec 16, 2018·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Berthold Huppertz
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Andreas Margraf, Markus Sperandio
Jul 14, 2019·Cellular Immunology·Nazihah HusnaYingrou Tan
Dec 15, 2020·Trends in Molecular Medicine·Lena Claesson-WelshDonald M McDonald
Mar 4, 2021·Current Opinion in Hematology·Andreia PenaCláudio Areias Franco
Jul 15, 2021·Advanced Science·Johannes ReblingDaniel Razansky

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Methods Mentioned

BETA
light microscopy
transmission electron microscopy
imaging techniques
transgenic

Software Mentioned

RVFS
KyPlot
sXYT
RVDM
Excel
MPLSM
fXYT

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