Intravital imaging of a pulmonary endothelial surface layer in a murine sepsis model

Biomedical Optics Express
Inwon ParkPilhan Kim

Abstract

Direct intravital imaging of an endothelial surface layer (ESL) in pulmonary microcirculation could be a valuable approach to investigate the role of a vascular endothelial barrier in various pathological conditions. Despite its importance as a marker of endothelial cell damage and impairment of the vascular system, in vivo visualization of ESL has remained a challenging technical issue. In this work, we implemented a pulmonary microcirculation imaging system integrated to a custom-design video-rate laser scanning confocal microscopy platform. Using the system, a real-time cellular-level microscopic imaging of the lung was successfully performed, which facilitated a clear identification of individual flowing erythrocytes in pulmonary capillaries. Subcellular level pulmonary ESL was identified in vivo by fluorescence angiography using a dextran conjugated fluorophore to label blood plasma and the red blood cell (RBC) exclusion imaging analysis. Degradation of ESL width was directly evaluated in a murine sepsis model in vivo, suggesting an impairment of pulmonary vascular endothelium and endothelial barrier dysfunction.

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Citations

Jan 13, 2019·The European Respiratory Journal·Inwon ParkPilhan Kim
Mar 10, 2020·Cellular Microbiology·Mariana De NizCarolina Agop-Nersesian
Jul 28, 2019·Intensive Care Medicine Experimental·Claire H MastersonJohn G Laffey
Mar 1, 2019·Biorheology·Axel R Pries
Feb 2, 2021·Frontiers in Cell and Developmental Biology·Nazli Alizadeh-TabriziChristian Lehmann
May 19, 2021·Translational Vision Science & Technology·Jehwi JeonPilhan Kim

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