Intravital Imaging of B Cell Responses in Lymph Nodes

Methods in Molecular Biology
Stefano SammicheliMatteo Iannacone

Abstract

Humoral immune responses depend on B cells encountering antigen (Ag) in lymph nodes (LNs) draining infection sites, getting activated, interacting with different cells, proliferating and differentiating into antibody (Ab)-secreting cells. Each of these events occurs in distinct LN sub-compartments, requiring the migration of B cells from niche to niche in a fast and tightly coordinated fashion. While some of the rules that characterize B cell behavior in secondary lymphoid organs have been elucidated at the population level, we have only limited knowledge of the precise dynamics of B cell interactions with different kinds of LN cells at the single-cell level. Here, we describe in detail an intravital microscopy technique that allows the analysis of B cell dynamic behavior in the popliteal lymph node of anesthetized mice at high spatial and temporal resolution. A detailed understanding of the spatiotemporal dynamics of B cells within secondary lymphoid organs may lead to novel, rational vaccine strategies aimed at inducing rapid and long-lived humoral immune responses.

Citations

Feb 7, 2020·Viral Immunology·Eleonora Sala, Mirela Kuka
Nov 18, 2021·Immunological Reviews·Dominik Schienstock, Scott N Mueller
Nov 24, 2021·Immunological Reviews·Mirela Kuka, Matteo Iannacone

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