Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability

Methods in Molecular Biology
Yookyung JungCharles P Lin

Abstract

The bone marrow is a unique microenvironment where blood cells are produced and released into the circulation. At the top of the blood cell lineage are the hematopoietic stem cells (HSC), which are thought to reside in close association with the bone marrow vascular endothelial cells (Morrison and Scadden, Nature 505:327-334, 2014). Recent efforts at characterizing the HSC niche have prompted us to make close examinations of two distinct types of blood vessel in the bone marrow, the arteriolar vessels originating from arteries and sinusoidal vessels connected to veins. We found the two vessel types to exhibit different vascular permeabilites, hemodynamics, cell trafficking behaviors, and oxygen content (Itkin et al., Nature 532:323-328, 2016; Spencer et al., Nature 508:269-273, 2014). Here, we describe a method to quantitatively measure the permeability and hemodynamics of arterioles and sinusoids in murine calvarial bone marrow using intravital microscopy.

Citations

Nov 24, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·JungMo Kim, Maria Gabriele Bixel
Aug 6, 2021·PloS One·Juwell W WuCharles P Lin

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