Intravital imaging of thymopoiesis reveals dynamic lympho-epithelial interactions

Immunity
Isabell Hess, Thomas Boehm

Abstract

T cell development occurs in the thymus. The thymic microenvironment attracts hematopoietic progenitors, specifies them toward the T cell lineage, and orchestrates their differentiation and egress into the periphery. The anatomical location of the thymus and the intrauterine development of mouse embryos have so far precluded a direct visualization of the initial steps of thymopoiesis. Here, we describe transgenic zebrafish lines enabling the in vivo observation of thymopoiesis. The cell-autonomous proliferation of thymic epithelial cells, their morphological transformation into a reticular meshwork upon contact with hematopoietic cells, and the multiple migration routes of thymus-settling cells could be directly visualized. The unexpectedly dynamic thymus homing process is chemokine driven and independent of blood circulation. Thymocyte development appears to be completed in less than 4 days. Our work establishes a versatile model for the in vivo observation and manipulation of thymopoiesis.

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Citations

Sep 21, 2013·Nature Reviews. Immunology·Thomas Boehm, Jeremy B Swann
Dec 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Dongyuan MaFeng Liu
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Isabell HessThomas Boehm
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