Intravital microscopic interrogation of peripheral taste sensation

Scientific Reports
Myunghwan ChoiSeok Hyun Yun

Abstract

Intravital microscopy is a powerful tool in neuroscience but has not been adapted to the taste sensory organ due to anatomical constraint. Here we developed an imaging window to facilitate microscopic access to the murine tongue in vivo. Real-time two-photon microscopy allowed the visualization of three-dimensional microanatomy of the intact tongue mucosa and functional activity of taste cells in response to topically administered tastants in live mice. Video microscopy also showed the calcium activity of taste cells elicited by small-sized tastants in the blood circulation. Molecular kinetic analysis suggested that intravascular taste sensation takes place at the microvilli on the apical side of taste cells after diffusion of the molecules through the pericellular capillaries and tight junctions in the taste bud. Our results demonstrate the capabilities and utilities of the new tool for taste research in vivo.

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Citations

Oct 23, 2018·Advanced Materials·Yei Hwan JungTae-Il Kim
Mar 29, 2020·Sensors·Elena von MolitorTiziana Cesetti
Mar 27, 2020·Chemical Senses·Alastair J M Reith, Charles Spence
Oct 9, 2020·Pflügers Archiv : European journal of physiology·Elena von MolitorTiziana Cesetti
Apr 16, 2021·Chemical Senses·Quan T NguyenMarco Tizzano

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Methods Mentioned

BETA
fluorescence microscopy
fluorescence imaging
electrophoresis

Software Mentioned

Matlab
Fiji
ImageJ

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