Abstract
Four antennae-specific proteins (AaegOBP1, AaegOBP2, AaegOBP3, and AaegASP1) were isolated from the yellow fever mosquito, Aedes aegypti and their full-length cDNAs were cloned. RT-PCR indicated that they are expressed in female and, to a lesser extent, in male antennae, but not in control tissues (legs). AaegOBP1 and AaegOBP3 showed significant similarity to previously identified mosquito odorant-binding proteins (OBPs) in cysteine spacing pattern and sequence. Two of the isolated proteins have a total of eight cysteine residues. The similarity of the spacing pattern of the cysteine residues and amino acid sequence to those of previously identified olfactory proteins suggests that one of the cysteine-rich proteins (AaegOBP2) is an OBP. The other (AaegASP1) did not belong to any group of known OBPs. Structural analyses indicate that six of the cysteine residues in AaegOBP2 are linked in a similar pattern to the previously known cysteine pairing in OBPs, i.e., Cys-24-Cys-55, Cys-51-Cys-104, Cys-95-Cys-113. The additional disulfide bridge, Cys-38-Cys-125, knits the extended C-terminal segment of the protein to a predicted alpha2-helix. As indicated by circular dichroism (CD) spectra, the extra rigidity seems to prevent the predic...Continue Reading
Citations
Aug 12, 2005·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·M Rützler, L J Zwiebel
Nov 22, 2008·Journal of Chemical Ecology·Hong-Liang LiBing-Gan Lou
Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Allison F Carey, John R Carlson
Jan 8, 2013·Genome Biology and Evolution·Malini ManoharanBernard Offmann
Aug 30, 2008·PloS One·Walter S LealAndré Furtado
Jul 17, 2009·PloS One·Julien Pelletier, Walter S Leal
Dec 4, 2009·PloS One·Ney Ribeiro LeiteGlaucius Oliva
Nov 3, 2010·PloS One·Wei XuWalter S Leal
Aug 13, 2013·PloS One·Yuhua DengXiaoguang Chen
Nov 11, 2010·Journal of Insect Science·Meryem S Sengul, Zhijian Tu
Oct 2, 2012·Annual Review of Entomology·Walter S Leal
Apr 9, 2009·Journal of Medical Entomology·Peter ArmbrusterXiumei Zhao
May 12, 2012·Biochimica Et Biophysica Acta·Wen-Ting ChuHong-Xing Zhang
Sep 25, 2008·Insect Molecular Biology·M S Sengul, Z Tu
Nov 17, 2009·Insect Molecular Biology·M S Sengul, Z Tu
Mar 2, 2016·PloS One·Patrícia C Bezerra-SilvaDaniela M A F Navarro
Jun 16, 2007·Chemical Senses·Zainulabeuddin Syed, Walter S Leal
Feb 12, 2015·F1000Research·Gabriel M Leal, Walter S Leal
Feb 12, 2015·F1000Research·Gabriel M Leal, Walter S Leal