Intrinsic biological activity of the thrombospondin structural homology repeat in connective tissue growth factor

The Journal of Endocrinology
Zhen-Yue Tong, David R Brigstock

Abstract

Connective tissue growth factor (CCN2) is a 349-residue mosaic protein that contains four structural modules (modules 1-4), which are presumptive domains for interactions with regulatory binding proteins and receptors. Module 3, corresponding to residues 199-243, is a thrombospondin structural homology repeat (TSR) and is flanked by regions that are highly susceptible to proteolytic cleavage. To test whether CCN2 module 3 (CCN2(3)) has intrinsic biological properties, it was produced recombinantly in Escherichia coli (E. coli) and examined for its effects on the function of hepatic stellate cells (HSC), the principal fibrogenic cell type in the liver. CCN2(3) stimulated dose-dependent HSC adhesion and activity of p42/p44 mitogen activated protein kinase, the latter of which was antagonized by blocking the activity of focal adhesion kinase. HSC adhesion to immobilized CCN2(3) was attributed to binding interactions with cell surface integrin alpha6beta1. As assessed by RT-PCR or Western blotting, CCN2(3) stimulated production of fibronectin and pro-collagen type IV(alpha5), both of which are downstream components of HSC-mediated fibrogenesis and which are constituents of high density matrix in fibrotic lesions. These data show th...Continue Reading

Citations

May 6, 2011·The Journal of Biological Chemistry·Kenneth P HolbournK Ravi Acharya
Mar 6, 2008·The Journal of Clinical Endocrinology and Metabolism·Ayad A JaffaUNKNOWN Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications Study Group
Oct 5, 2011·Critical Reviews in Eukaryotic Gene Expression·John A ArnottSteven N Popoff
May 16, 2008·Journal of Cell Communication and Signaling·Takashi NishidaKaren M Lyons
May 16, 2008·Journal of Cell Communication and Signaling·Mark R GrayJohn J Castellot
May 9, 2009·Journal of Cell Communication and Signaling·Kenneth P HolbournK Ravi Acharya
Nov 11, 2010·Journal of Cell Communication and Signaling·Bethanie Morrison, Mary Lou Cutler
Mar 20, 2012·Journal of Cell Communication and Signaling·Véronique Ouellet, Peter M Siegel
Dec 21, 2010·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Margaret MarkiewiczMaria Trojanowska
Apr 22, 2010·Journal of Cellular and Molecular Medicine·Guangcun Huang, David R Brigstock
Sep 10, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Liya PiBryon E Petersen
Apr 10, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Cassie M TranMakarand V Risbud
Jul 30, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Georgina S ButlerChristopher M Overall
May 21, 2011·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Amélie DendoovenRoel Goldschmeding
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Zihao ChenBao-Ting Zhang
Jan 12, 2021·Journal of Cell Communication and Signaling·Roos J LeguitRoel Goldschmeding

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