Intrinsic cardiac catecholamines help maintain beating activity in neonatal rat cardiomyocyte cultures

Pediatric Research
Aruna R NatarajanSteven N Ebert

Abstract

In the present study, we identify intrinsic cardiac adrenergic (ICA) cells in the neonatal rat heart using immunofluorescent histochemical staining techniques with antibodies that specifically recognize the major enzymes in the catecholamine biosynthetic pathway. ICA cells are most concentrated near the endocardial surface of ventricular myocardium, but are also found sporadically throughout the heart. In primary cultures of neonatal rat cardiomyocytes, ICA cells are closely associated with clusters of cardiomyocytes. To investigate a potential role for intrinsically produced catecholamines, we recorded beating rates in the presence and absence of the catecholamine-depleting agent reserpine or the adrenergic receptor blockers prazosin and timolol using videomicroscopy and photodiode sensors. Our results show that beating rates slow significantly when endogenous catecholamines are depleted or when their action is blocked with either a beta- or an alpha1-adrenergic receptor antagonist. These data indicate that intrinsic cardiac catecholamines help to maintain beating rates in neonatal rat cardiomyocyte cultures via stimulation of alpha1- and beta-adrenergic receptors. This information should help to increase our understanding of ...Continue Reading

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Citations

Jul 19, 2015·European Journal of Pharmacology·Elizângela S OliveiraRosana A Bassani
Jun 12, 2012·Biochemical and Biophysical Research Communications·Candice BakerSteven N Ebert
Oct 8, 2005·Biology of the Neonate·Rashmi ChandraDona M Chikaraishi
Jan 2, 2010·Journal of Neurosurgery. Pediatrics·Oumar SackoFranck-Emmanuel Roux
Apr 4, 2017·Advanced Healthcare Materials·Dmitry KireevAndreas Offenhäusser
Aug 19, 2020·Open Biology·Alexander Grassam-RoweMing Lei
Sep 12, 2016·Journal of Cardiovascular Development and Disease·Anna M D VéghMonique R M Jongbloed
Dec 18, 2014·American Journal of Physiology. Endocrinology and Metabolism·Candice N BakerSteven N Ebert

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