Intrinsic enzymatic crosslinking and maturation of the in situ pellicle

Archives of Oral Biology
Christian HannigM Hannig

Abstract

The acquired enamel pellicle is a proteinaceous layer formed on all solid substrata exposed to the oral cavity. It has been supposed that the pellicle undergoes maturation after protein adsorption. The aim of the present study was to investigate enzyme activities with an impact on intrinsic maturation processes in in situ formed pellicles. Bovine enamel specimens were exposed to the oral cavity in six subjects to allow in situ pellicle formation over 3, 30 and 120 min. The slabs were fixed on the buccal and palatal surfaces of individual splints fixed with silicone impression material. After rinsing with deionised water, the pellicle samples were tested fluorimetrically for transglutaminase, protease and elastase activity. Phosphatase activities were tested photometrically. Separate samples were used for each of the enzymes tested. Transglutaminase was detected in in situ pellicle (16.7+/-21.2 mU/cm(2)) as was alkaline phosphatase activity (0.87+/-0.99 mU/cm(2)). For both enzymes, there was no correlation of enzyme activities with time or localisation of pellicle formation. Acidic phosphatase- and protease-activities were not detectable. Only traces of elastase activity were found in 57% of the samples. Transglutaminase and pho...Continue Reading

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Citations

Sep 24, 2008·Clinical Oral Investigations·Christian HannigMatthias Hannig
Jan 13, 2009·Clinical Oral Investigations·Christian Hannig, Matthias Hannig
Dec 8, 2009·Clinical Oral Investigations·Christian HannigMatthias Hannig
Jan 20, 2011·Clinical Oral Investigations·Susanne ProkschAli Al-Ahmad
Nov 10, 2012·Journal of Applied Oral Science : Revista FOB·Marília Afonso Rabelo BuzalafMelissa Thiemi Kato
Mar 27, 2009·Archives of Oral Biology·C HannigM Hannig
Mar 10, 2009·Archives of Oral Biology·Walter L Siqueira, Frank G Oppenheim
May 13, 2008·Journal of Dentistry·Christian HannigMatthias Hannig
Apr 22, 2008·Archives of Oral Biology·Christian HannigMatthias Hannig
Sep 1, 2015·European Journal of Oral Sciences·Susann GrychtolChristian Hannig
Jul 6, 2014·Case Reports in Dentistry·Kavitha RamarK Ieshwaryah
Jun 13, 2014·Colloids and Surfaces. B, Biointerfaces·Hannah L GibbinsGuy H Carpenter
Jan 14, 2017·Colloids and Surfaces. B, Biointerfaces·Judith DeliusThomas Hofmann
Dec 15, 2016·Scientific Reports·Hardy SchweigelFalk Schwendicke
Mar 11, 2015·Clinical Oral Investigations·Elżbieta PaszynskaChristian Hannig
Nov 6, 2013·The Analyst·Nunzianda FrascioneBarbara Daniel
Nov 11, 2020·Caries Research·Tatiana MartiniMarília Afonso Rabelo Buzalaf

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