Intrinsic factor-mediated attachment of vitamin B12 to brush borders and microvillous membranes of hamster intestine

The Journal of Clinical Investigation
R M DonaldsonJ S Trier

Abstract

Hamster intrinsic factor (IF) preparations markedly enhanced the uptake of (57)cobalt-labeled cyanocobalamin (B(12)-(57)Co) by brush borders and microvillous membranes isolated from villous absorptive cells obtained from the distal but not the proximal half of hamster intestine. A similar effect was observed with rat and rabbit IF preparations, but IF preparations obtained from man, dog, and hog were ineffective. After fractionation of hamster IF preparations by gel filtration or ion exchange chromatography, the extent to which each fraction enhanced B(12)-(57)Co uptake by brush borders correlated closely with the vitamin B(12) binding capacity of the fraction. IF-mediated attachment of B(12)-(57)Co to brush borders occurred rapidly, was not diminished by removal of glucose or oxygen from the incubation medium, and was not significantly altered when incubation temperatures were reduced from 37 degrees C to 7 degrees C. Marked reduction in uptake occurred, however, in the absence of divalent cations. IF enhanced B(12)-(57)Co uptake by brush borders isolated from the proximal half of the intestine when these proximal brush borders were preincubated with supernatant fluid obtained after centrifugation of homogenates of distal inte...Continue Reading

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