Intrinsic fluorescence as an analytical probe of virus-like particle assembly and maturation

Biochemical and Biophysical Research Communications
Simon J HanslipRobert J Falconer

Abstract

The assembly and maturation of the human papillomavirus (HPV) virus-like particle (VLP) has been monitored by measuring the intrinsic fluorescence intensity using excitation at 290nm and emission at 350nm. The assay was validated to eliminate error due to photo-bleaching, adsorption, and precipitation. Intrinsic fluorescence intensity dropped during both assembly and maturation phases. The decrease during assembly had a second-order dependence on capsomere concentration, as previously observed using light scattering. During post-assembly structural modification the decrease had a first-order dependence on capsomere concentration. Intrinsic fluorescence spectroscopy complements light scattering methodologies for monitoring assembly and enables kinetics of maturation to be observed. The role of environmental factors such as the presence of oxidized glutathione in facilitation of faster and more complete maturation was monitored in real time. Intrinsic fluorescence is a rugged methodology that could be applied to monitoring VLP assembly and maturation unit operations during HPV vaccine manufacturing.

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Citations

Dec 16, 2010·Journal of Molecular Recognition : JMR·Robert J Falconer, Brett M Collins
Jun 18, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Louise T ChowBettie M Steinberg
Sep 10, 2014·Expert Review of Anti-infective Therapy·Eric J Ryndock, Craig Meyers
Dec 18, 2012·The Journal of Physical Chemistry. B·Stefano C MeligaRobert J Falconer

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