Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model

Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics
Seon-Ah ChongIsabelle Niespodziany

Abstract

Understanding the mechanisms of epileptogenesis is essential to develop novel drugs that could prevent or modify the disease. Neuroinflammation has been proposed as a promising target for therapeutic interventions to inhibit the epileptogenic process that evolves from traumatic brain injury. However, it remains unclear whether cytokine-related pathways, particularly TNFα signaling, have a critical role in the development of epilepsy. In this study, we investigated the role of innate inflammation in an in vitro model of post-traumatic epileptogenesis. We combined organotypic hippocampal slice cultures, representing an in vitro model of post-traumatic epilepsy, with multi-electrode array recordings to directly monitor the development of epileptiform activity and to examine the concomitant changes in cytokine release, cell death, and glial cell activation. We report that synchronized ictal- and interictal-like activities spontaneously evolve in this culture. Dynamic changes in the release of the pro-inflammatory cytokines IL-1β, TNFα, and IL-6 were observed throughout the culture period (3 to 21 days in vitro) with persistent activation of microglia and astrocytes. We found that neutralizing TNFα with a polyclonal antibody signifi...Continue Reading

References

Aug 23, 1990·The New England Journal of Medicine·N R TemkinH R Winn
Sep 20, 1984·The Journal of Comparative Neurology·J Zimmer, B H Gähwiler
Nov 5, 1997·Trends in Neurosciences·B H GähwilerS M Thompson
Jan 8, 1999·Brain Research·R Gutiérrez, U Heinemann
Aug 18, 2000·The European Journal of Neuroscience·M G De SimoniA Vezzani
Mar 23, 2002·Science·Eric C BeattieRobert C Malenka
Mar 26, 2003·Cell Death and Differentiation·H WajantP Scheurich
Jun 6, 2003·Cytokine & Growth Factor Reviews·Paul W DempseyGenhong Cheng
Apr 22, 2004·Pharmacology, Biochemistry, and Behavior·Giovambattista De SarroAngela De Sarro
Apr 27, 2005·Annals of Neurology·Silvia BalossoAnnamaria Vezzani
Jul 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Liliana BernardinoJens Zimmer
Oct 22, 2005·Epilepsy & Behavior : E&B·L James Willmore
Apr 4, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Krishnan SriramJames P O'Callaghan
Aug 22, 2006·Experimental Neurology·Akitaka YamamotoDavid C Henshall
Oct 13, 2006·Science's STKE : Signal Transduction Knowledge Environment·James O McNamaraA Soren Leonard
Jun 8, 2007·Brain & Development·Mitsumasa FukudaYasushi Ishida
Feb 21, 2008·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Bernhard T BauneHeinrich Koerner
Aug 30, 2008·The Journal of Physiology·Caroline Le DuigouRichard Miles
Nov 7, 2008·Journal of the Royal Society, Interface·Cristina SavinMichael Meyer-Hermann
Dec 23, 2008·Journal of Neuroscience Methods·Yevgeny BerdichevskyMartin L Yarmush
Aug 19, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Adel HelmyPeter J A Hutchinson
Nov 16, 2010·Journal of Clinical Neurophysiology : Official Publication of the American Electroencephalographic Society·J Dyhrfjeld-JohnsenK J Staley

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Electrophoresis
PCR
PCA

Software Mentioned

MC Rack
ImageJ
qbase
Rad CFX Manager
Labview
qbase +
geNormplus
Bio

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Yevgeny BerdichevskyKevin J Staley
Journal of Clinical Neurophysiology : Official Publication of the American Electroencephalographic Society
J Dyhrfjeld-JohnsenKevin J Staley
European Journal of Medicinal Chemistry
Carmela SpagnuoloGian Luigi Russo
© 2022 Meta ULC. All rights reserved