PMID: 6165581Mar 1, 1981Paper

Intrinsic molecules in fluid phospholipid bilayers. Fluorescence probe studies

European Journal of Biochemistry
W HoffmannD Chapman

Abstract

Fluorescence probe data using 1,6-diphenyl-1,3,5-hexatriene for various concentrations of intrinsic molecules (cholesterol, gramicidin A amd cytochrome oxidase) within fluid lipid bilayers have been examined. The polarization value increases with increasing concentration of intrinsic molecule and then approaches a limiting value. Empirical curve-fitting of the experimental data, change of polarization with concentration, shows that each system can be fitted approximately by an exponential curve. A theory has been constructed based upon the assumption that only one intrinsic molecule need be adjacent to a fluorescent probe molecule to affect its motion drastically. The change in probe motion then depends upon the probability p of all positions next to a lipid chain being free of intrinsic molecules. The value of the probability p has been calculated and it is shown that (formula: see text) depending on whether the intrinsic molecule spans the lipid bilayer or not. The approximation p = e-Mx gives a good fit to the data for all x, thereby explaining the observed phenomenological fit. The fluorescent probe data is interpreted to show that protein-protein contacts increase as the intrinsic protein concentration increases within the...Continue Reading

References

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Dec 1, 1978·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A Seelig, J Seelig
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J DavoustP F Devaux
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Citations

Dec 1, 1987·Journal of Bioenergetics and Biomembranes·A G Lee
Apr 1, 1992·Lipids·D BorchmanM C Yappert
Mar 1, 1984·Chemistry and Physics of Lipids·A ManjonJ L Iborra
Nov 1, 1988·Chemistry and Physics of Lipids·T NishiyaT M Chang
Dec 26, 2001·Biophysical Journal·María del Mar Martínez-SenacJuan C Gómez-Fernández
Apr 7, 1987·Biochemistry·A L MacDonald, D A Pink
Aug 21, 2008·The Journal of Physical Chemistry. B·Alessio AusiliJuan C Gómez-Fernández
Jan 22, 1988·Biochimica Et Biophysica Acta·D A Pink, A L MacDonald
Jul 27, 1983·Biochimica Et Biophysica Acta·E K MurrayD Chapman
Nov 30, 1982·Biochemical and Biophysical Research Communications·M EsfahaniT M Devlin
Jul 6, 2012·Biochemistry·Juan H BolivarAnthony G Lee

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