Intrinsic radiosensitivity of human pancreatic tumour cells and the radiosensitising potency of the nitric oxide donor sodium nitroprusside

British Journal of Cancer
V N VerovskiG A Storme

Abstract

A panel of eight human pancreatic tumour cell lines displayed high intrinsic radioresistance, with mean inactivation doses between 2.4 and 6.5 Gy, similar to those reported for melanoma and glioblastoma. The radiosensitising potency of sodium nitroprusside, a bioreductive nitric oxide donor, was assessed in a model of metabolism-induced hypoxia in a cell micropellet. Sodium nitroprusside at 0.1 mM revealed a radiosensitising effect with an overall enhancement ratio of 1.9 compared with 2.5 for oxygen. Radiosensitising activity correlated with the enhancement of single-strand DNA breakage caused by radiation. In suspensions with cell densities of between 3% and 30% (v/v), the half-life of sodium nitroprusside decreased from 31 to 3.2 min, suggesting a value of around 1 min for micropellets. Despite this variation, the radiosensitising activity was similar in micropellets and in diluted cell suspensions. S-nitroso-L-glutathione was found to possess radiosensitising activity, consistent with a possible role of natural thiols in the storing of radiobiologically active nitric oxide adducts derived from sodium nitroprusside. As measured by a nitric oxide-specific microsensor, activation of sodium nitroprusside occurred by bioreductio...Continue Reading

Citations

May 6, 2011·Cancer Chemotherapy and Pharmacology·Simendra Singh, Alok K Gupta
Jul 1, 2020·Experimental Biology and Medicine·Sukanya RoySanjukta Chakraborty
Feb 22, 2001·Radiation Research·H MatsumotoE Kano
Mar 19, 2005·The British Journal of Radiology·S Anoopkumar-DukieT J Conere
Jul 5, 2005·Expert Opinion on Investigational Drugs·S P Fricker
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Max HecklerAstrid Weyerbrock
Jan 24, 2019·Cancers·Hui WangMark De Ridder
Jan 31, 2021·Vaccines·Joel MintzHimanshu Arora
Apr 11, 2002·Chemical Reviews·Anthony R Butler, Ian L Megson
Jun 14, 2000·Archives of Biochemistry and Biophysics·L A MacMillan-CrowJ A Thompson
Mar 1, 1998·Environmental Toxicology and Pharmacology·H BabichL Blau
Sep 24, 2004·International Journal of Radiation Oncology, Biology, Physics·Mark De RidderGuy A Storme
May 14, 2008·Nitric Oxide : Biology and Chemistry·Mark De RidderGuy Storme
Sep 16, 2010·Free Radical Biology & Medicine·Simone ReuterBharat B Aggarwal

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