Intrinsic resistance of oligodendrocytes to prion infection

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Marco PrinzAdriano Aguzzi

Abstract

Within the CNS, the normal form of cellular prion protein (PrP(C)) is expressed on neurons, oligodendrocytes, and astrocytes. The contribution of these cell types to prion replication and pathogenesis is unclear. To assess the role of oligodendrocytes, we expressed PrP(C) under the control of the myelin basic protein (MBP) promoter in mice lacking endogenous PrP(C). PrP(C) was detected in oligodendrocytes and Schwann cells but not in neurons and astrocytes. MBP-PrP mice never developed scrapie after intracerebral, intraperitoneal, or intraocular challenge with scrapie prions. Transgenic brains did not contain protease-resistant prion protein and did not transmit scrapie when inoculated into PrP(C)-overexpressing indicator mice. To investigate whether prion spread within the CNS depends on oligodendrocytic PrP(C), we implanted PrP(C)-overexpressing neuroectodermal grafts into MBP-PrP brains. After intraocular prion inoculation, none of the grafts showed spongiform encephalopathy or prion infectivity. Hence oligodendrocytes do not support cell-autonomous prion replication, establishment of subclinical disease, and neural spread of prions. Prion resistance sets oligodendrocytes aside from both neurons and astrocytes.

Citations

Jan 13, 2005·Acta Neuropathologica·Ursula UnterbergerHerbert Budka
Dec 23, 2008·Nature Neuroscience·Claire E Le PichonStuart Firestein
Oct 31, 2008·Journal of Virology·Jacob I AyersJason C Bartz
Jan 1, 2012·ISRN Veterinary Science·Peter HedlinScott Napper
Aug 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Magdalini PolymenidouAdriano Aguzzi
May 12, 2012·AJNR. American Journal of Neuroradiology·H LeeI Prohovnik
Dec 3, 2009·The Journal of Cell Biology·Hristelina IlievaDon W Cleveland
Oct 20, 2015·Journal of the Neurological Sciences·Daniela Veber, Giuseppe Scalabrino
Dec 14, 2011·Glia·Frank W Pfrieger, Michal Slezak
Mar 29, 2008·The American Journal of Pathology·Nicolas GenoudAdriano Aguzzi
Sep 23, 2014·The International Journal of Biochemistry & Cell Biology·Giuseppe ScalabrinoGiovanni Tredici
Nov 26, 2011·Experimental Neurology·Giuseppe ScalabrinoGiovanni Tredici
Feb 3, 2015·Frontiers in Cell and Developmental Biology·Akikazu Sakudo, Takashi Onodera
Jan 14, 2017·Acta Neuropathologica·Sebastian Brandner, Zane Jaunmuktane
Oct 20, 2017·Journal of Virology·Takeshi YamasakiMotohiro Horiuchi
Nov 23, 2017·The Journal of Experimental Medicine·Adriano Aguzzi, Yingjun Liu
Jan 26, 2010·Nature Neuroscience·Juliane BremerAdriano Aguzzi
Aug 21, 2018·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Ivan Fernández-VegaIsidro Ferrer
Aug 23, 2017·Scientific Reports·Jae Young LeeSteven Petratos
Jul 21, 2017·The Journal of Cell Biology·Guiliana Soraya Victoria, Chiara Zurzolo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.