PMID: 9166404Jun 2, 1997Paper

Intrinsic signals in the unique domain target p56(lck) to the plasma membrane independently of CD4

The Journal of Cell Biology
M J BijlmakersM Marsh

Abstract

In T lymphocytes, the Src-family protein tyrosine kinase p56(lck) (Lck) is mostly associated with the cytoplasmic face of the plasma membrane. To determine how this distribution is achieved, we analyzed the location of Lck in lymphoid and in transfected nonlymphoid cells by immunofluorescence. We found that in T cells Lck was targeted correctly, independently of the cell surface proteins CD4 and CD8 with which it interacts. Similarly, in transfected NIH-3T3 fibroblasts, Lck was localized at the plasma membrane, indicating that T cell-specific proteins are not required for targeting. Some variation in subcellular distribution was observed when Lck was expressed in HeLa and MDCK cells. In these cells, Lck associated with both the plasma membrane and the Golgi apparatus, while subsequent expression of CD4 resulted in the loss of Golgi-associated staining. Together, these data indicate that Lck contains intrinsic signals for targeting to the plasma membrane. Furthermore, delivery to this site may be achieved via association with exocytic transport vesicles. A mutant Lck molecule in which the palmitoylation site at cysteine 5 was changed to lysine (LC2) localized to the plasma membrane and the Golgi region in NIH3T3 cells. However, ...Continue Reading

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Citations

Dec 8, 1998·European Journal of Immunology·A Pelchen-MatthewsM Marsh
Dec 14, 2002·Trends in Cell Biology·Marie-José Bijlmakers, Mark Marsh
Jul 22, 2008·Nature Cell Biology·Teodoro PulvirentiAlberto Luini
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