Intrinsic viscosity of ovomucoid in random coil conformation.

International Journal of Peptide and Protein Research
F Ahmad, A Salahuddin

Abstract

Ovomucoid is denatured by concentrated solutions of guanidine hydrochloride. The intrinsic viscosities of the glycoprotein in 6 M guanidine hydrochloride in the absence and presence of beta-mercaptoethanol were found to be 8.1 and 16.0 ml/g, respectively. Ovomucoid with disulphide bonds reduced exists in linear random coil conformation. However, the intrinsic viscosity of the randomly coiled protein was less than that predicted from the empirical equations describing the molecular weight dependence of intrinsic viscosities of random coil proteins in 6 M guanidine hydrochloride. On excluding the carbohydrate content of the protein, which is theoretically justified, the calculated intrinsic viscosity interestingly became closer to the measured one. The temperature dependence of the intrinsic viscosity of ovomucoid in linear random coil conformation was studied in the temperature range, 25-55 degrees. The features of the intrinsic viscosity-temperature profile are not comparable with those exhibited by other linear random coil proteins in 6 M guanidine hydrochloride.

References

Dec 1, 1973·The Biochemical Journal·A A Ansari, A Salahuddin
Sep 12, 1972·Biochemistry·J J O'Malley, J L Weaver

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Citations

Jun 15, 1977·European Journal of Biochemistry·A WaheedA Salahuddin
Jan 1, 1978·The International Journal of Biochemistry·F Ahmad, P McPhie

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