Intrinsically Disordered Proteins as Important Players during Desiccation Stress of Soybean Radicles

Journal of Proteome Research
Yun LiuVladimir N Uversky

Abstract

Intrinsically disordered proteins (IDPs) play a variety of important physiological roles in all living organisms. However, there is no comprehensive analysis of the abundance of IDPs associated with environmental stress in plants. Here, we show that a set of heat-stable proteins (i.e., proteins that do not denature after boiling at 100 °C for 10 min) was present in R0mm and R15mm radicles (i.e., before radicle emergence and 15 mm long radicles) of soybean (Glycine max) seeds. This set of 795 iTRAQ-quantified heat-stable proteins contained a high proportion of wholly or highly disordered proteins (15%), which was significantly higher than that estimated for the whole soybean proteome containing 55,787 proteins (9%). The heat-stable proteome of soybean radicles that contain many IDPs could protect lactate dehydrogenase (LDH) during freeze-thaw cycles. Comparison of the 795 heat-stable proteins in the R0mm and R15mm soybean radicles revealed that many of these proteins changed abundance during seedling growth with 170 and 89 proteins being more abundant in R0mm and R15mm, respectively. KEGG analysis identified 18 proteins from the cysteine and methionine metabolism pathways and nine proteins from the phenylpropanoid biosynthesis p...Continue Reading

References

Nov 5, 1999·Journal of Molecular Biology·P E Wright, H J Dyson
Feb 25, 2000·Journal of Molecular Biology·J A ZitzewitzC R Matthews
Nov 28, 2000·Proteins·P RomeroA K Dunker
Mar 27, 2001·Pacific Symposium on Biocomputing·R M WilliamsA K Dunker
May 31, 2001·Journal of Molecular Graphics & Modelling·A K DunkerZ Obradovic
Jun 13, 2001·Plant Physiology·K GallardoD Job
Jan 11, 2002·European Journal of Biochemistry·Vladimir N Uversky
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Vladimir N Uversky
May 23, 2002·Biochemistry·A Keith DunkerZoran Obradović
Oct 9, 2002·Trends in Biochemical Sciences·Peter Tompa
Oct 17, 2002·Journal of Molecular Biology·Lilia M IakouchevaA Keith Dunker
Nov 7, 2002·Advances in Protein Chemistry·H Jane Dyson, Peter E Wright
Dec 19, 2003·Nucleic Acids Research·Rolf ApweilerLai-Su L Yeh
Feb 13, 2004·Nucleic Acids Research·Lilia M IakouchevaA Keith Dunker
Feb 9, 2005·Biochemistry·Christopher J OldfieldA Keith Dunker
Feb 19, 2005·Current Opinion in Structural Biology·Anthony L Fink
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Mar 8, 2005·Journal of Bioinformatics and Computational Biology·Kang PengZoran Obradovic
Aug 12, 2005·Journal of Molecular Recognition : JMR·Vladimir N UverskyA Keith Dunker
Sep 15, 2005·Biochemistry·Christopher J OldfieldA Keith Dunker
Oct 11, 2005·Journal of Proteome Research·Marc S CorteseA Keith Dunker
Oct 13, 2005·The FEBS Journal·A Keith DunkerVladimir N Uversky
Apr 19, 2006·BMC Bioinformatics·Kang PengZoran Obradovic
Dec 13, 2006·Biophysical Journal·Predrag RadivojacA Keith Dunker
Sep 4, 2007·Protein Science : a Publication of the Protein Society·Seung-Wook ChiKyou-Hoon Han
Mar 5, 2008·BMB Reports·Soonbok KwonDongbin Lim
Mar 6, 2008·BMC Genomics·Michaela Hundertmark, Dirk K Hincha
May 10, 2008·Journal of Proteome Research·Oleg PaliyA Keith Dunker
Aug 15, 2008·Journal of Proteome Research·Wilfred H TangSean L Seymour
Dec 20, 2008·PLoS Computational Biology·Agnes Tóth-PetróczyMonika Fuxreiter
May 5, 2009·PLoS Computational Biology·Bálint MészárosZsuzsanna Dosztányi
Aug 4, 2009·Molecular Cell·Natalie De JongeRemy Loris

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Citations

Jul 3, 2019·Genes·Jesús Alejandro Zamora-BriseñoLuis Carlos Rodríguez-Zapata
Sep 10, 2020·Cell Stress & Chaperones·Jesús Alejandro Zamora-BriseñoLuis Carlos Rodríguez-Zapata
Jun 4, 2018·Cell Stress & Chaperones·Jesús Alejandro Zamora-BriseñoLuis Carlos Rodríguez Zapata
Aug 7, 2020·International Journal of Molecular Sciences·Liyi ChenGuobao Liu
Nov 3, 2020·International Journal of Biological Macromolecules·Fangmei TanYun Liu
Aug 8, 2021·International Journal of Molecular Sciences·Orarat GinsawaengEllen Zuther

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