Introducing a mouse model of brain death

Journal of Neuroscience Methods
G PomperB K Podesser

Abstract

Experimental animal models of brain death increasing intracranial pressure (ICP) by inflating an intracranial placed balloon-catheter are well established and used in transplant-associated studies. Our aim was to develop an experimental mouse model of brain death (BD) and to compare explosive and gradual brain death induction under ICP monitoring. We therefore induced BD in female OF-1 mice by injecting 40 microl saline every 5 min into an intracranial placed balloon increasing ICP rapidly [BD ex, n=7], or gradually [BD grad, n=7] with 20 microl volume every 5 min under electroencephalogram (EEG) and ICP monitoring until BD occurred. The major criterion for BD was a flat-line-EEG, confirmed by cessation of spontaneous respiration and maximally dilated and fixed pupils. ICP, central activity and heart rate were continuously monitored during the entire 6h follow-up. In sham-operated controls [control, n=7] a burr hole was drilled but no balloon inserted. The BD groups showed equal ICP levels at the time of BD. Both groups had increased heart rates (HR) 15 min after BD, HR decreased to 402+/-29.39 bpm (beats per minute) [BD ex] and 409.33+/-26.46 bpm [BD grad] (n.s. vs. control) by 30 min after the inflation of the balloon, but on...Continue Reading

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Citations

Jun 24, 2014·Journal of the American College of Surgeons·Jason W SmithPaul J Matheson
Feb 12, 2013·The Journal of Surgical Research·Shana WautersDirk E M Van Raemdonck
Aug 30, 2016·Transplant International : Official Journal of the European Society for Organ Transplantation·Jamila KremerBruno K Podesser
Mar 10, 2020·Transplantation·Louise E See HoeJohn F Fraser

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