DOI: 10.1101/499715Dec 19, 2018Paper

Introducing THOR, a model microbiome for genetic dissection of community behavior

BioRxiv : the Preprint Server for Biology
Gabriel L LozanoJo Handelsman

Abstract

The quest to manipulate microbiomes has intensified, but many microbial communities have proven recalcitrant to sustained change. Developing model communities amenable to genetic dissection will underpin successful strategies for shaping microbiomes by advancing understanding of community interactions. We developed a model community with representatives from three dominant rhizosphere taxa: the Firmicutes, Proteobacteria, and Bacteroidetes. We chose Bacillus cereus as a model rhizosphere Firmicute and characterized twenty other candidates, including hitchhikers that co-isolated with B. cereus from the rhizosphere. Pairwise analysis produced a hierarchical interstrain-competition network. We chose two hitchhikers - Pseudomonas koreensis from the top tier of the competition network and Flavobacterium johnsoniae from the bottom of the network to represent the Proteobacteria and Bacteroidetes, respectively. The model community has several emergent properties - induction of dendritic expansion of B. cereus colonies by either of the other members and production of more robust biofilms by the three members together than individually. Moreover, P. koreensis produces a novel family of alkaloid antibiotics that inhibit growth of F. johns...Continue Reading

Related Concepts

Bacillus cereus
Cell Growth
Tissue Dissection
Gene Therapy
Genome
Microbial Biofilms
Plant alkaloid
Shapes
Proteobacteria
Analysis

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