PMID: 6170060Jul 1, 1981Paper

Introduction by molecular cloning of artifactual inverted sequences at the 5' terminus of the sense strand of bovine parathyroid hormone cDNA

Proceedings of the National Academy of Sciences of the United States of America
C A WeaverB Kemper

Abstract

To study the structure and function of the gene for parathyroid hormone, we obtained recombinant plasmids containing bovine parathyroid hormone cDNA. The nucleotide sequence at the 5' terminus (relative to the sense strand) of the cDNA insert in a recombinant plasmid, pPTHi4, was different from that previously reported for the bovine parathyroid hormone cDNA insert of another recombinant plasmid, pPTHm1 [Kronenberg, H. M., McDevitt, B. F., Majzoub, J. A., Nathans, J., Sharp, P. A., Potts, J. T., Jr. & Rich, A. (1979) Proc. Natl. Acad. Sci. USA 76, 4981-4985]. The first 50 nucleotides of the pPTHm1 insert were an inverted complement of nucleotides 2-51 of the pPTHi4 insert. the cDNA insert of another plasmid, pPTHi8, contained a sequence identical to nucleotides 2-51 of the pPTHi4 insert but also contained an additional 42 bases at the 5' terminus. The first 41 bases of the pPTHi8 insert were an inverted repeat of an internal sequence of the pPTHi4 insert corresponding to nucleotides 184-224. Restriction endonuclease analysis of pPTHi8 indicated that the internal sequence corresponding to this region was retained. The nucleotide sequence of a restriction fragment hybridized to parathyroid hormone mRNA and extended toward the 5' ...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L Villa-KomaroffW Gilbert
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H M KronenbergA Rich
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S J ChanD F Steiner
Nov 10, 1979·Nucleic Acids Research·R I RichardsH M Goodman

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Citations

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G N HendyA Rich
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·O J YooK L Agarwal
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T J VasicekH M Kronenberg
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T G AhnM A Levine
Jan 1, 1983·DNA·M CombE Herbert
May 25, 1982·Nucleic Acids Research·M Karin, R I Richards
Dec 20, 1982·Nucleic Acids Research·S C BockR M Lawn
Apr 10, 1982·Nucleic Acids Research·D E BlackburnJ M Rosen
Nov 1, 1982·Molecular and Cellular Endocrinology·C A WeaverB Kemper
Oct 14, 1983·Biochemical and Biophysical Research Communications·K Motojima, T Oka
Jan 1, 1986·CRC Critical Reviews in Biochemistry·B Kemper
Oct 1, 1989·Hypertension·R J ParmerD T O'Connor

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