Introduction of a 20 kHz Nd:YVO4 laser into a hybrid quadrupole time-of-flight mass spectrometer for MALDI-MS imaging

Analytical and Bioanalytical Chemistry
Paul J TrimMalcolm R Clench

Abstract

A commercial hybrid quadrupole time-of-flight mass spectrometer has been modified for high-speed matrix-assisted laser desorption ionisation (MALDI) imaging using a short-pulse optical technology Nd:YVO(4) laser. The laser operating in frequency-tripled mode (lambda = 355 nm) is capable of delivering 1.5-ns pulses of energy at up to 8 microJ at 5-10 kHz and 3 microJ at 20 kHz. Experiments to improve beam homogeneity and reduce laser speckle by mechanical vibration of the fibre-optic laser delivery system are reported along with data from trial and tissue imaging experiments using the modified instrument. The laser appeared to yield best results for MALDI-MS imaging experiments when operating at repetition rates 5-10 kHz. Combining this with raster imaging allowed images of rat brain sections to be recorded in 37 min. Similarly, images of the distribution of peptides in "on-tissue" digest experiments from tumour tissues were recorded in 1 h and 30 min rather than the 8-h acquisition time previously used. A brief investigation of targeted protein analysis/imaging by multiple reaction monitoring experiments "on-tissue" is reported. A total of 26 transitions were recorded over a 3-s cycle time and images of abundant proteins were s...Continue Reading

References

Aug 13, 2005·Journal of the American Society for Mass Spectrometry·John C JurchenJonathan V Sweedler
Feb 2, 2006·Analytical Chemistry·E MoskovetsB L Karger
May 24, 2006·Journal of Mass Spectrometry : JMS·Armin HolleJens Höhndorf

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Citations

May 24, 2011·Analytical and Bioanalytical Chemistry·Philippa J HartMalcolm R Clench
Mar 22, 2013·Analytical and Bioanalytical Chemistry·Rory T Steven, Josephine Bunch
May 9, 2013·Histochemistry and Cell Biology·Andreas Römpp, Bernhard Spengler
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Jeffrey M Spraggins, Richard M Caprioli
Mar 5, 2013·Journal of the American Society for Mass Spectrometry·Rory T StevenJosephine Bunch
May 28, 2013·Journal of the American Society for Mass Spectrometry·Rory T StevenJosephine Bunch
Aug 24, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mohammadreza ShariatgorjiPer E Andrén
Jan 15, 2013·The Analyst·David Gode, Dietrich A Volmer
Jun 18, 2011·Bioanalysis·David BonnelJonathan Stauber
Mar 22, 2014·Analytical and Bioanalytical Chemistry·Klaus Dreisewerd
May 14, 2014·Journal of the American Society for Mass Spectrometry·David M G AndersonKevin L Schey
Apr 24, 2015·Journal of the American Society for Mass Spectrometry·Jeffrey M SpragginsRichard M Caprioli
Aug 16, 2016·Bioanalysis·Andrea E SteuerThomas Kraemer
Nov 1, 2016·Journal of the American Society for Mass Spectrometry·Marcel WiegelmannJens Soltwisch
Sep 23, 2016·Journal of the American Society for Mass Spectrometry·Boone M PrenticeRichard M Caprioli
Jan 23, 2016·Electrophoresis·Núria CanelaLluís Arola
Nov 12, 2013·Vascular and Endovascular Surgery·Joseph ShalhoubAlun H Davies
Sep 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yoshinori FujimuraHirofumi Tachibana
Jul 8, 2015·Journal of Mass Spectrometry : JMS·Boone M PrenticeRichard M Caprioli
Jan 18, 2020·Rapid Communications in Mass Spectrometry : RCM·Keiko KuwataYasuhide Naito
Feb 22, 2011·Rapid Communications in Mass Spectrometry : RCM·Alicia L RichardsSarah Trimpin
Apr 30, 2016·Proteomics·Lisa DeiningerSimona Francese
Jun 16, 2011·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Eleanor Q BlatherwickDaniel J Weston
Jun 1, 2013·Journal of Mass Spectrometry : JMS·Leesa S FergusonSimona Francese
Jun 30, 2018·Current Opinion in Chemical Biology·Brendan PrideauxVéronique Dartois
Apr 6, 2021·Journal of Mass Spectrometry : JMS·Kermit K Murray
Aug 13, 2017·Journal of the American Society for Mass Spectrometry·Bryn FlindersRon M A Heeren
Apr 30, 2013·Analytical Chemistry·S FranceseM R Clench
Oct 8, 2013·Analytical Chemistry·Beixi Wang, Sarah Trimpin
Dec 20, 2012·Analytical Chemistry·Judith M FonvilleJosephine Bunch
Feb 15, 2018·Analytical Chemistry·Boone M PrenticeJeffrey M Spraggins
Feb 5, 2015·ACS Chemical Neuroscience·Iván ManuelRafael Rodríguez-Puertas

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