Introduction of a citrus blight-associated gene into Carrizo citrange [Citrus sinensis (L.) Osbc. x Poncirus trifoliata (L.) Raf.] by Agrobacterium-mediated transformation

Plant Cell Reports
M KayimK S Derrick

Abstract

The protein p12 accumulates in leaves of trees with citrus blight (CB), a serious decline of unknown cause. The function of p12 is not known, but sequence analysis indicates it may be related to expansins. In studies to determine the function of p12, sense and antisense constructs were used to make transgenic Carrizo citrange using an Agrobacterium-mediated transformation system. Homogeneous beta-glucuronidase+ (GUS+) sense and antisense transgenic shoots were regenerated using kanamycin as a selective agent. Twenty-five sense and 45 antisense transgenic shoots were in vivo grafted onto Carrizo citrange for further analyses. In addition, 20 sense and 18 antisense shoots were rooted. The homogeneous GUS+ plants contained either the p12 sense or antisense gene (without the intron associated with the gene in untransformed citrus) as shown by PCR and Southern blotting. Northern blots showed the expected RNA in the sense and antisense plants. A protein of identical size and immunoreactivity was observed in seven of nine sense plants but not in nine antisense or non-transgenic plants. At the current stage of growth, there are no visual phenotypic differences between the transgenic and non-transgenic plants. Selected plants will be bu...Continue Reading

References

Feb 14, 1992·Analytical Biochemistry·P Chomczynski
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S GowdaR J Shepherd
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·T Y ShcherbanD J Cosgrove
Nov 27, 1998·Nature Biotechnology·Y Chen, J M Wilson
Nov 27, 1998·Nature Biotechnology·L ChenC M Fauquet
Dec 23, 1998·Plant Molecular Biology·T L CeccardiK S Derrick
Nov 1, 1992·The Plant Cell·S McQueen-MasonD J Cosgrove
Nov 10, 2001·Annual Review of Phytopathology·K S Derrick, L W Timmer
Mar 14, 2002·Plant Physiology·Yi LiSimon J McQueen-Mason

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Citations

Jul 18, 2012·Plant Cell Reports·Luzia Yuriko MiyataFrancisco de Assis Alves Mourão Filho
Jan 1, 2009·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Sandeepa Singh, Manchikatla V Rajam
Jan 10, 2009·Molecular Plant-microbe Interactions : MPMI·Aïda AzaiezArmand Séguin
Aug 29, 2013·TheScientificWorldJournal·Dicle DonmezYesim Yalcin Mendi

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