Introduction of a disulfide bond into a cationic lipid enhances transgene expression of plasmid DNA

Biochemical and Biophysical Research Communications
F Tang, J A Hughes

Abstract

We have introduced a convenient method of synthesis for disulfide-containing cationic lipids. The lipid, 1,2-dioleoyl-sn-glycero-3-succinyl-2-hydroxyethyl disulfide ornithine conjugate (DOGSDSO), was synthesized and used to prepare liposomes in combination with DOPE. The rationale behind the selection of the disulfide bond was to produce a lipid which could be selectively destabilized within the cytosol of the cell. The disulfide bond of DOGSDSO was shown to be cleaved by reductive media leading to destabilization of the liposome/DNA complex, thus increasing the release of pDNA compared to a non-disulfide-containing analog. The introduction of a disulfide bond increases the transfection activity using model animal cell lines. The transfection activity and toxicity of DOTAP, DOGSDSO and its analog in three cell lines were compared. The amount of transgene (e.g. luciferase) produced with the use of DOGSDSO/DOPE was greater than that of DOTAP/DOPE and up to 50 times more than that of its non-disulfide analog. The results indicate disulfide-containing cationic liposomes may act as excellent vectors for gene transfection.

Citations

Oct 10, 2008·Journal of the American Chemical Society·Winston OngRobin L McCarley
Jun 8, 2001·The Biochemical Journal·B WetzerD Scherman
Jun 17, 1999·Alcoholism, Clinical and Experimental Research·C Di CampliM A Zern
Nov 3, 2010·Nanomedicine·Inmaculada PosadasValentín Ceña
Oct 1, 2013·Applied Microbiology and Biotechnology·M A S ToledoA R Azzoni
Sep 19, 2009·Genetica·Romain LabasBruno Pitard
Jan 1, 2001·Journal of Liposome Research·P Chancham, J A Hughes
Oct 20, 2006·Journal of Drug Targeting·Devika Soundara Manickam, David Oupický
Sep 15, 2010·Expert Opinion on Drug Delivery·Lin Zhu, Ram I Mahato
Mar 25, 2010·Expert Opinion on Drug Delivery·Mary Caldorera-MooreKrishnendu Roy
Nov 6, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Daniele PezzoliGabriele Candiani
Dec 14, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oleg O MarkovMarina A Zenkova
Apr 3, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Conchita Tros de IlarduyaNejat Düzgüneş
Jan 12, 2010·Chemistry and Physics of Lipids·N Madhusudhana Rao
Oct 3, 2008·Biotechnology Journal·Zhijun WangLi Jin
Nov 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubiao ZhangZongshi Li
Jul 13, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mathias G SvahnLars J Brandén
Mar 15, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Christopher M JewellDavid M Lynn
May 31, 2011·Biochimie·Xiao-Xiang ZhangMark W Grinstaff
Oct 4, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Burcu S AytarDavid M Lynn
Oct 10, 2006·Bioscience Reports·N Madhusudhana Rao, Vijaya Gopal
Jul 14, 2016·Journal of Medicinal Chemistry·Kyle E GieslerDennis C Liotta
Sep 28, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Edmund J NiedzinskiMichael H Nantz
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Zhaohua HuangFrancis C Szoka
Feb 24, 2001·Bioorganic & Medicinal Chemistry Letters·C JacopinJ Herscovici
Feb 28, 2014·Advanced Healthcare Materials·Ming WangQiaobing Xu
Oct 6, 2006·Medicinal Research Reviews·Priya Prakash Karmali, Arabinda Chaudhuri
Mar 10, 2005·The Journal of Gene Medicine·Richard W Horobin, Volkmar Weissig
Aug 27, 2009·Organic & Biomolecular Chemistry·Colin ByrneRaphael Darcy
Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
Oct 1, 2010·Advanced Materials·Sonja BauhuberAchim Göpferich
Sep 12, 2002·Journal of Physiology and Biochemistry·A RochaJ M Coll
Jan 16, 2007·Chemical Communications : Chem Comm·Richard G UhlVladimir Sidorov
Nov 6, 2013·Biological & Pharmaceutical Bulletin·Kosuke KandaHitoshi Sasaki
Dec 22, 1999·The American Journal of Physiology·X TangM I Phillips
Sep 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Aysha S AnsariHasan Uludağ
Jan 15, 2019·European Journal of Medicinal Chemistry·Mingyuan LiHervé Galons

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